Xeno-free, defined, feeder-free medium for maintenance of undifferentiated human ES and iPS cells
Figure 1. Morphology of hPSCs Maintained in TeSR™2 is Comparable to hPSCs Cultured in mTeSR™1
(A,B) Undifferentiated human ES (H9) cells cultured on Corning® Matrigel® matrix in TeSR™2 retain the prominent nucleoli and high nuclear-to-cytoplasm ratio characteristic of this cell type. Densely packed cells and multi-layering are apparent when cells are ready to be passaged. (C,D) H9 cells cultured under the same conditions in mTeSR™1 exhibit comparable morphology.
Figure 2. Fold and Cumulative Aggregate Expansion in TeSR™2
Graph shows the average fold expansion per passage ± SEM obtained for human ES and iPS cells cultured in mTeSR™1 (brown) or TeSR™2 (red) with Corning® Matrigel® over 10 passages. Expansion was determined by counting the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded.
Note: This data is representative of cultures passaged after 5-6 days in culture, lower expansion should be expected if using shorter culture times.
Figure 3. hESCs Cultured in TeSR™2 Are Pluripotent
H9 cells were cultured for 11 passages in TeSR™2, then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown.
Figure 4. Human Pluripotent Stem Cells Cultured in TeSR™2 Retain Expression of Undifferentiated Cell Markers
Histogram analysis for H9 human ES and WLS-1C human iPS cells characterized using flow cytometry for undifferentiated cell markers (SSEA-3 and OCT3/4) after passaging in TeSR™2 for 21 passages (WLS-1C) and 18 passages (H9) respectively (filled histogram = sample, hollow histogram = secondary antibody only).
Figure 5. Human ES Cells Cultured Long-Term in TeSR™2 Retain Normal Karyotype
Chromosomal analysis of H9 hES cells cultured in TeSR™2 for 12 passages shows that normal karyotype is retained during passaging.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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