STEMtaq™ Hot Start DNA Polymerase Master Mix Kit

Hot Start DNA polymerase master mix and nuclease-free water for PCR reactions at room temperature

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From: 75 USD

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Hot Start DNA polymerase master mix and nuclease-free water for PCR reactions at room temperature
From: 75 USD

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Overview

STEMtaq™ Hot Start DNA Polymerase Master Mix Kit, which includes Hot Start master mix and nuclease-free water, reliably and consistently amplifies a wide range of PCR templates in a convenient master mix format that allows for room temperature setup. The enzyme is bound to a proprietary antibody that blocks activity until the initial denaturation step (94 - 95℃ for 2 minutes) of the PCR re-activates the enzyme. Hot Start PCR can in some cases eliminate or minimize primer-dimers and secondary products for some targets, and potentially improve yield. STEMtaq™ Hot Start DNA Polymerase Master Mix is a 2X concentrated, ready-to-use solution containing the Hot Start version of the STEMtaq™ DNA polymerase, dNTPs, MgCl₂, and reaction buffer. STEMtaq™ Hot Start DNA polymerase has 5’ to 3’ exonuclease activity and produces PCR fragments with a 3’ A overhang.
Components:
STEMtaq™ Hot Start DNA Polymerase Master Mix Kit - 100 Reactions (Catalog #79013)
• STEMtaq™ Hot Start DNA Polymerase Master Mix, 2 x 1.25 mL
• Nuclease-Free Water, 2 x 1.25 mL
 
STEMtaq™ Hot Start DNA Polymerase Master Mix Kit - 1000 Reactions (Catalog #79013.1)
• STEMtaq™ Hot Start DNA Polymerase Master Mix, 25 mL
• Nuclease-Free Water, 25 mL
Subtype:
Genome Editing
Species:
Human; Mouse; Rat
Brand:
STEMtaq
Area of Interest:
Disease Modeling

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STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.