STEMtaq™ Hot Start DNA Polymerase Master Mix Kit, which includes Hot Start master mix and nuclease-free water, reliably and consistently amplifies a wide range of PCR templates in a convenient master mix format that allows for room temperature setup. The enzyme is bound to a proprietary antibody that blocks activity until the initial denaturation step (94 - 95℃ for 2 minutes) of the PCR re-activates the enzyme. Hot Start PCR can in some cases eliminate or minimize primer-dimers and secondary products for some targets, and potentially improve yield. STEMtaq™ Hot Start DNA Polymerase Master Mix is a 2X concentrated, ready-to-use solution containing the Hot Start version of the STEMtaq™ DNA polymerase, dNTPs, MgCl₂, and reaction buffer. STEMtaq™ Hot Start DNA polymerase has 5’ to 3’ exonuclease activity and produces PCR fragments with a 3’ A overhang.
STEMtaq™ Hot Start DNA Polymerase Master Mix Kit - 100 Reactions (Catalog #79013)
• STEMtaq™ Hot Start DNA Polymerase Master Mix, 2 x 1.25 mL
• Nuclease-Free Water, 2 x 1.25 mL
STEMtaq™ Hot Start DNA Polymerase Master Mix Kit - 1000 Reactions (Catalog #79013.1)
• STEMtaq™ Hot Start DNA Polymerase Master Mix, 25 mL
• Nuclease-Free Water, 25 mL
Human; Mouse; Rat
Area of Interest:
Technical Bulletin Genome Editing with Direct Cas9 RNP Delivery Design Considerations
Sale of STEMtaq is covered under at least US Patent No. 6,242,235, Australian Patent No. 761757, Canadian Patent No. 2,335,153, Chinese Patent No. ZL99808861.7, Hong Kong Patent No. HK 1040262, Japanese Patent No. 3673175, European Patent No. 1088060, other patents pending, and all applicable STEMCELL Terms and Conditions of Sale. STEMtaq can be used for basic PCR experiments in research use only applications without the need to acquire a license from STEMCELL or payment of royalty fees to STEMCELL.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.