StemSep™ Human CD14 Positive Selection Kit

Immunomagnetic column-based positive selection kit

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StemSep™ Human CD14 Positive Selection Kit

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1 x 109 cells
Catalog #14758
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Overview

The StemSep™ Human CD14 Positive Selection Kit is designed to isolate CD14+ cells from fresh or previously frozen peripheral blood mononuclear cells by positive selection. Desired cells are targeted with Tetrameric Antibody Complexes recognizing CD14 and dextran-coated magnetic particles. Labeled cells are separated using commercially available columns and StemSep™ magnets. The CD14 antigen is strongly expressed on monocytes and weakly expressed on granulocytes. It is also expresed on most tissue macrophages.
Components:
  • StemSep™ Human CD14 Positive Selection Kit (Catalog #14758)
    • StemSep™ Human CD14 Positive Selection Cocktail, 1 mL
    • StemSep™ Magnetic Colloid, 1.5 mL
Magnet Compatibility:
• StemSep™ Magnet (Catalog #11030, 11050, 11060, 11070) or a magnet with the strength of at least 0.6 Tesla
• Commercially available positive selection columns
Subtype:
Cell Isolation Kits
Cell Type:
Monocytes
Species:
Human
Sample Source:
PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
StemSep
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(1)

Data and Publications

Data

Typical StemSep™ CD14 Selection Profile

Figure 1. Typical StemSep™ CD14 Selection Profile

The expected CD14+ content of the enriched fraction is 97.5 ± 0.5% with expected recoveries of 58 ± 9%.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.