STEMscript™ cDNA Synthesis Kits

For reverse transcription of RNA before qPCR or RNA sequencing

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more

STEMscript™ cDNA Synthesis Kits

For reverse transcription of RNA before qPCR or RNA sequencing

STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
1 Kit
589 USD
Catalog # 79003

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing

STEMscript™ cDNA Synthesis Kit with Random Primers
1 Kit
589 USD
Catalog # 79004

For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing

What's Included

STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers (Catalog #79003)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Oligo(dT) Primers, 2 x 200 µL
 
STEMscript™ cDNA Synthesis Kit with Random Primers (Catalog #79004)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Random Primers, 2 x 200 µL
New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more
Products for Your Protocol

Overview

STEMscript™ cDNA Synthesis Kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water. This kit can be used to reverse transcribe total RNA, mRNA, or synthetic transcript RNA, and is required for gene expression analyses such as qPCR and RNA sequencing.

STEMscript™ cDNA Synthesis Kit is available with either oligo(dT) or random primers. Oligo(dT) primers anneal to the poly(A) tails of eukaryotic mRNAs, whereas random primers are used for reverse transcription of RNA without poly(A) tails, such as rRNA, tRNA, degraded RNA, RNA isolated from prokaryotic sources, and RNA with known secondary structures.
Species
Human, Mouse, Rat
Brand
STEMscript

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
79003
Lot #
All
Language
English
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
79004
Lot #
All
Language
English

Educational Materials (1)

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