STEMscript™ cDNA Synthesis Kits

For reverse transcription of RNA before qPCR or RNA sequencing

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STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing

1 Kit
Catalog #79003
475 USD

STEMscript™ cDNA Synthesis Kit with Random Primers

For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing

1 Kit
Catalog #79004
475 USD

Required Products

Overview

STEMscript™ cDNA Synthesis Kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water. This kit can be used to reverse transcribe total RNA, mRNA, or synthetic transcript RNA, and is required for gene expression analyses such as qPCR and RNA sequencing.

STEMscript™ cDNA Synthesis Kit is available with either oligo(dT) or random primers. Oligo(dT) primers anneal to the poly(A) tails of eukaryotic mRNAs, whereas random primers are used for reverse transcription of RNA without poly(A) tails, such as rRNA, tRNA, degraded RNA, RNA isolated from prokaryotic sources, and RNA with known secondary structures.
Components:
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers (Catalog #79003)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Oligo(dT) Primers, 2 x 200 µL
 
STEMscript™ cDNA Synthesis Kit with Random Primers (Catalog #79004)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Random Primers, 2 x 200 µL
Species:
Human; Mouse; Rat
Brand:
STEMscript

Scientific Resources

Educational Materials

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STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.