STEMdiff™ Trilineage Differentiation Kit

Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers

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STEMdiff™ Trilineage Differentiation Kit

Functional assay kit to assess pluripotency by directed differentiation of human ES and iPS cells to all three germ layers

1 Kit
Catalog #05230
460 USD

Required Products

Overview

The STEMdiff™ Trilineage Differentiation Kit provides a simple culture assay to functionally validate the ability of new or established human embryonic stem (ES) and induced pluripotent stem (iPS) cell lines to differentiate to the three germ layers: ectoderm, mesoderm, and endoderm. This kit includes specialized, complete media and monolayer-based protocols to perform parallel in vitro directed differentiation experiments for each germ layer, clearly and reproducibly establishing trilineage differentiation potential within one week. STEMdiff™ Trilineage Differentiation Kit is intended to be an endpoint assay and is not optimized for the generation of cells for downstream differentiation or other applications. STEMdiff™ Trilineage Differentiation Kit has been optimized to assess cells maintained in mTeSR™1.
Advantages:
• Reproducible differentiation to all three germ layers across multiple pluripotent cell lines
• Easy-to-interpret assay results
• Complete, defined culture media
• Standardized, one-week protocol
Components:
  • STEMdiff™ Trilineage Differentiation Kit (Catalog #05230)
    • STEMdiff™ Trilineage Ectoderm Medium, 175 mL
    • STEMdiff™ Trilineage Mesoderm Medium, 100 mL
    • STEMdiff™ Trilineage Endoderm Medium, 100 mL
Subtype:
Specialized Media
Cell Type:
Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Characterization; Differentiation; Functional Assay; Phenotyping
Brand:
STEMdiff
Area of Interest:
Stem Cell Biology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.
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