STEMdiff™ Sensory Neuron Maturation Kit

Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells

STEMdiff™ Sensory Neuron Maturation Kit

Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells

From: 349 USD
Catalog #
100-0684_C
Maturation kit for generation of functional sensory neurons from human iPS cell-derived sensory neuronal precursor cells

Product Advantages

⦁ Reduce animal usage by eliminating costly dorsal root ganglia explant workflows
⦁ Mature and maintain sensory neurons seamlessly following STEMdiff™ Sensory Neuron Differentiation Kit
⦁ Streamline PSC-derived sensory neuron generation with a simple, easy-to-use media format
⦁ Obtain physiologically relevant results with integrated BrainPhys™ media supporting neuronal activity and maturation

What's Included

  • BrainPhys™ Neuronal Medium, 100 mL (Catalog #05797)
  • STEMdiff™ Sensory Neuron Maturation Supplement, 25 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Product Information Sheet.

Overview

Generate functional, human-specific sensory neurons in a dish with this easy-to-use culture system. STEMdiff™ Sensory Neuron Maturation Kit is the final step in a workflow beginning from human pluripotent stem cells (hPSCs) and leading to a population of sensory neurons that can be used for drug discovery and pain research applications. After obtaining PSC-derived neural crest cells (NCCs) with STEMdiff™ Neural Crest Differentiation Kit, use the serum-free STEMdiff™ Sensory Neuron Differentiation Kit to generate sensory neuron precursors. STEMdiff™ Sensory Neuron Maturation Kit can then be used to maintain and mature the sensory neuron precursors. The resulting cells are BRN3A-positive and more than 70% TUJ1-positive. With the physiological glucose and osmolarity conditions provided by BrainPhys™ as the basal medium, the neurons exhibit activity in response to sensory ligands and changes in temperature.
Subtype
Specialized Media
Cell Type
Neural Cells, PSC-Derived, Neurons
Species
Human
Application
Cell Culture, Characterization, Differentiation, Functional Assay, Immunocytochemistry, Phenotyping
Brand
BrainPhys, STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
Formulation
Serum-Free

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0684
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0684
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0684
Lot #
All
Language
English

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Experimental Protocol for STEMdiff™ Sensory Neuron Differentiation and Maturation Kits

Figure 1. Schematic for the STEMdiff™ Sensory Neuron Culture System Protocol

Sensory neurons can be generated in 7 days from sensory neuron precursors after 6 days in STEMdiff™ Sensory Neuron Differentiation Medium. For the differentiation of sensory neuron precursors from hPSC-derived neural crest cells, see the PIS.

Figure 2. STEMdiff™ Sensory Neuron Kits Promote Differentiation Across Multiple Embryonic Stem and Induced Pluripotent Stem Cell Lines

NCCs generated from hPSCs in mTeSR™ Plus using the STEMdiff™ Neural Crest Differentiation Kit were differentiated and matured to sensory neurons using the STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. (A) Sensory neurons were generated after hPSC-derived NCCs were cultured with the STEMdiff™ Sensory Neuron Differentiation Kit for 6 days and then the STEMdiff™ Sensory Neuron Maturation Kit for 6 days. The resulting cultures contain a population of cells expressing sensory neuron markers peripherin (green) and BRN3A (red) along with (B) neuronal marker class III β-tubulin (TUJ1, red). (C) Midbrain neuron controls generated with STEMdiff™ Midbrain Neuron Differentiation and Maturation Kits do not have detectable peripherin (green) or BRN3A (red) expression, although they express (D) neuronal marker class III β-tubulin (TUJ1, red). Nuclei are labeled with DAPI (blue). Human ES and iPS cell lines were maintained in either mTeSR™1, TeSR™-E8™, or mTeSR™ Plus and differentiated with STEMdiff™ Neural Crest Differentiation Kit, followed by STEMdiff™ Sensory Neuron Differentiation and Maturation Kits. The percentage expression of (E) BRN3A+ and (F) TUJ1+ cells in the resulting cultures was quantified. This differentiation generated BRN3A+ sensory neurons (25.3% ± 6.9%, mean ± SEM; n=7 cell lines, 3 - 23 replicates per condition) that expressed neuronal marker class III β-tubulin (TUJ1; 90.3% ± 4.1%, mean ± SEM; n=4 cell lines, 3 - 12 replicates per condition). Numbers are % positive over total DAPI in a tiled image. NCCs = neural crest cells; hPSCs = human pluripotent stem cells; ES = embryonic stem; iPS = induced pluripotent stem

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