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Data and Publications
(A) WLS-4D1 (iPS) cells maintained in TeSR™-E8™ (Catalog #05940) were dissociated, plated as single cells, and cultured in STEMdiff™ Neural Induction Medium (Catalog #05835) for differentiation into neural progenitor cells. Cells were fixed after 7 days in culture and labeled with Anti-PAX6 Antibody and Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by fluorochrome-conjugated secondary antibodies. Cells that have adopted a neural fate are positive for PAX6 (red) whereas undifferentiated iPS cells are positive for OCT4 (OCT3) (green).
(B) Neural progenitor cells derived from H9 (ES) cells using STEMdiff™ Neural Induction Medium were cultured in STEMdiff™ Neural Progenitor Medium (Catalog #05833). Cells were fixed and labeled with Anti-Human SOX1 Antibody, Clone EPR4766, and Anti-Human Nestin, Clone 10C2, followed by fluorochrome-conjugated secondary antibodies. Cells positive for the neural progenitor cell markers SOX1 (red) and Nestin (green) are evident.