STEMdiff™ Human Neural Progenitor Antibody Panel

Antibody panel for the identification of neural progenitor cells and undifferentiated human ES/iPS cells

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STEMdiff™ Human Neural Progenitor Antibody Panel

Antibody panel for the identification of neural progenitor cells and undifferentiated human ES/iPS cells

1 Kit
Catalog #69001
1,872 USD

Overview

STEMdiff™ Human Neural Progenitor Antibody Panel is suitable for immunocytochemical characterization of neural progenitor cells, including those generated from human embryonic stem (ES) and induced pluripotent stem (iPS) cells. The panel provides primary antibodies that are immunoreactive towards marker proteins highly expressed either by neural progenitor cells (Nestin, PAX6, SOX1) or by undifferentiated human ES and iPS cells (OCT4/OCT3). This panel may be used to monitor the generation of neural progenitor cells during differentiation of pluripotent stem cells and is also suitable for labeling primary cells and tissues. All antibodies have been validated for immunocytochemistry. Antibodies against Nestin, OCT4 (OCT3), and PAX6 may also be used for flow cytometry.
Subtype:
Primary Antibodies
Target Antigen:
Nestin; OCT4 (OCT3); PAX6; SOX1
Reactive Species:
Human
Conjugation:
Unconjugated
Host Species:
Mouse; Rabbit
Cell Type:
Pluripotent Stem Cells; Neural Cells, PSC-Derived
Application:
Immunofluorescence; Immunocytochemistry; Flow Cytometry
Area of Interest:
Neuroscience; Stem Cell Biology

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) WLS-4D1 (iPS) cells maintained in TeSR™-E8™ (Catalog #05940) were dissociated, plated as single cells, and cultured in STEMdiff™ Neural Induction Medium (Catalog #05835) for differentiation into neural progenitor cells. Cells were fixed after 7 days in culture and labeled with Anti-PAX6 Antibody and Anti-Human OCT4 (OCT3) Antibody, Clone 3A2A20, followed by fluorochrome-conjugated secondary antibodies. Cells that have adopted a neural fate are positive for PAX6 (red) whereas undifferentiated iPS cells are positive for OCT4 (OCT3) (green).
(B) Neural progenitor cells derived from H9 (ES) cells using STEMdiff™ Neural Induction Medium were cultured in STEMdiff™ Neural Progenitor Medium (Catalog #05833). Cells were fixed and labeled with Anti-Human SOX1 Antibody, Clone EPR4766, and Anti-Human Nestin, Clone 10C2, followed by fluorochrome-conjugated secondary antibodies. Cells positive for the neural progenitor cell markers SOX1 (red) and Nestin (green) are evident.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.