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STEMdiff™ Dopaminergic Neuron Differentiation Kit

Differentiation kit for generation of dopaminergic neuronal precursors from human ES and iPS cell-derived neural progenitor cells

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STEMdiff™ Dopaminergic Neuron Differentiation Kit

Differentiation kit for generation of dopaminergic neuronal precursors from human ES and iPS cell-derived neural progenitor cells

1 Kit
Catalog #08520
295 USD

Required Products

Overview

The STEMdiff™ Dopaminergic Neuron Differentiation Kit is used to generate dopaminergic neuronal precursors from neural progenitor cells derived from human pluripotent stem cells using the STEMdiff™ Neural Induction Medium (Catalog #05835) embryoid body protocol. The neuronal precursors generated can be matured using the STEMdiff™ Dopaminergic Neuron Maturation Kit (Catalog #08530) to produce a neuronal population containing midbrain dopaminergic neurons (15 - 30% TH-positive dopaminergic neurons; >90% class III β-tubulin-positive neurons; <10% GFAP-positive astrocytes). Cells derived using these products are versatile tools for modeling human neurological development and disease, drug screening, toxicity testing, and cell therapy validation.

NOTE: supplementation with human recombinant sonic hedgehog (SHH; Catalog #78065) is required.
Advantages:
• Defined and serum-free
• Supports efficient generation of dopaminergic neuronal precursors from hPSC-derived NPCs
• Precursors generated can be matured to functional dopaminergic neurons using the STEMdiff™ Dopaminergic Neuron Maturation Kit
• Optimized for differentiation of NPCs generated using the STEMdiff™ Neural Induction Medium embryoid body protocol
• Enables reproducible differentiation of NPCs derived from multiple human ES and iPS cell lines
Components:
  • STEMdiff™ Dopaminergic Neuron Differentiation Basal Medium, 100 mL
  • STEMdiff™ Dopaminergic Neuron Differentiation Supplement A, 8 mL
  • STEMdiff™ Dopaminergic Neuron Differentiation Supplement B, 2 mL
  • STEMdiff™ Dopaminergic Neuron Differentiation Supplement C, 300 µL
Subtype:
Specialized Media
Cell Type:
Dopaminergic Neurons; Neural Cells, PSC-Derived; Neural Stem and Progenitor Cells; Neurons; Pluripotent Stem Cells
Species:
Human
Application:
Cell Culture; Differentiation
Brand:
STEMdiff
Area of Interest:
Disease Modeling; Drug Discovery and Toxicity Testing; Neuroscience; Stem Cell Biology
Formulation:
Serum-Free

Scientific Resources

Educational Materials

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Figure 1. Downstream Differentiation of Neural Progenitor Cells to Dopaminergic Neurons

Neural Progenitor Cells derived from iPS cells (STiPS-M001 cell line) using the STEMdiff™ Neural Induction Medium embryoid body protocol were differentiated and matured to dopaminergic neurons by culture in STEMdiff™ Dopaminergic Neuron Differentiation Kit for 13 days and STEMdiff™ Dopaminergic Neuron Maturation Kit for 13 days. The dopaminergic neurons express the neuronal marker class III β-tubulin (red) and the dopaminergic neuronal marker tyrosine hydroxylase (TH, green).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.