STEMdiff™ Blood Vessel Organoid Kit

Media and supplements for differentiation of human pluripotent stem cells to blood vessel organoids
STEMdiff™ Blood Vessel Maturation Medium

Media for maturation of human pluripotent stem cells derived blood vessels organoids

1 Kit - Bottle
Catalog # 100-0658

Overview

Generate physiologically relevant blood vessel organoids—easily and with high yield—for your tissue repair and regeneration studies or to model vascular disease in vitro by using STEMdiff™ Blood Vessel Organoid Kit.

Use STEMdiff™ Blood Vessel Organoid Kit for efficient and reproducible generation of blood vessel organoids from human pluripotent stem cells (hPSCs) compared to homemade protocols, with the option to scale up for high-throughput screening in a 96-well format. Compared to homemade organoid culture media formulations, organoids generated with this easy-to-use kit develop extensive and physiologically relevant vascular networks and strong endothelial cell-pericyte interactions. Blood vessel organoids generated using this kit have CD31+/CD34+/CD144+/KDR+ endothelial cells and PDGFR-β+/CD146+/SMA+/NG-2+ pericytes.The simple protocol also eliminates the need for serum screening, labor-intensive protocol optimization, so you can spend more time on what matters—your experiments.

Use STEMdiff™ Blood Vessel Organoid Kit with mTeSR™ Plus or mTeSR™1 culture media for an easy transition from hPSCs to organoids. To differentiate hPSCs to endothelial cells, use our STEMdiff™ Endothelial Differentiation Kit.
Advantages
⦁ Generates physiologically relevant 3D blood vessel organoids ideal for disease modeling and drug discovery
⦁ Provides a standardized protocol with optimized reagents for efficient generation of hPSC-derived organoids
⦁ Supports high-throughput scalability in a 96-well format for drug discovery or drug testing
Components
  • STEMdiff™ Blood Vessel Organoid Aggregation Basal Medium, 60 mL
  • STEMdiff™ Blood Vessel Organoid Aggregation Supplement, 15 mL
  • STEMdiff™ Blood Vessel Organoid Induction Basal Medium, 100 mL
  • STEMdiff™ Blood Vessel Organoid Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Mesodermal Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Vascular Induction Supplement, 1 mL
  • STEMdiff™ Blood Vessel Organoid Maturation Medium, 100 mL (Catalog #100-0658)
Subtype
Specialized Media, Supplements
Cell Type
Endothelial Cells, Endothelial Cells, PSC-Derived
Species
Human
Application
Functional Assay, Organoid Culture, Toxicity Assay
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Endothelial Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMdiff™ Blood Vessel Maturation Medium
Catalog #
100-0658
Lot #
All
Language
English
Document Type
Manual
Product Name
STEMdiff™ Blood Vessel Organoid Kit
Catalog #
100-0651
Lot #
All
Language
English

Educational Materials (3)

Webinar
Therapeutic Application of Endothelial Colony-Forming Cells for Retinal Diseases
29:38
Therapeutic Application of Endothelial Colony-Forming Cells for Retinal Diseases
Scientific Poster
STEMdiff™ Endothelial: A New Culture Workflow for Efficient Derivation and Expansion of Endothelial Cells From Human Pluripotent Stem Cells
Scientific Poster
STEMdiff™ Blood Vessel Organoid Kit Supports Efficient Generation of Vascular Organoids from Human Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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