STEMdiff™ APEL™2-LI Medium

Defined, low-insulin, animal component-free medium for the differentiation of human ES and iPS cells

This product will be discontinued on December 16, 2022. Please refer to our STEMdiff™ kits for your differentiation needs.

STEMdiff™ APEL™2-LI Medium

Defined, low-insulin, animal component-free medium for the differentiation of human ES and iPS cells

From: 177 USD
Catalog #
05271_C
Defined, low-insulin, animal component-free medium for the differentiation of human ES and iPS cells

Product Advantages


  • Compatible with TeSR™-cultured ES and iPS cells

  • Compatible with adherent or EB culture differentiation protocols

  • Can be used to direct differentiation to a variety of cell lineages when specific cytokines or induction factors are added

This product will be discontinued on December 16, 2022. Please refer to our STEMdiff™ kits for your differentiation needs.

Overview

STEMdiff™ APEL™ 2-LI Medium is a fully defined, low-insulin (LI), serum-free and animal component-free medium for the differentiation of human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. It is based on the APEL formulation published by Dr. Andrew Elefanty and lacks undefined components such as protein-free hybridoma medium.

STEMdiff™ APEL™ 2-LI can be used in adherent or embryoid body (EB)-based protocols, such as with AggreWell™. Appropriate induction factors must be added before use. The low insulin content of this medium makes it particularly useful for differentiation to lineages in which insulin is a known inhibitor, such as cardiomyocyte.
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Species
Human
Application
Cell Culture, Differentiation
Brand
STEMdiff
Area of Interest
Drug Discovery and Toxicity Testing, Stem Cell Biology
Formulation
Animal Component-Free, Serum-Free

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
05271
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
05271
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications

Publications (1)

Coculturing with endothelial cells promotes in vitro maturation and electrical coupling of human embryonic stem cell-derived cardiomyocytes. Pasquier J et al. The Journal of heart and lung transplantation : the official publication of the International Society for Heart Transplantation 2017 JUN

Abstract

BACKGROUND Pluripotent human embryonic stem cells (hESC) are a promising source of repopulating cardiomyocytes. We hypothesized that we could improve maturation of cardiomyocytes and facilitate electrical interconnections by creating a model that more closely resembles heart tissue; that is, containing both endothelial cells (ECs) and cardiomyocytes. METHODS We induced cardiomyocyte differentiation in the coculture of an hESC line expressing the cardiac reporter NKX2.5-green fluorescent protein (GFP), and an Akt-activated EC line (E4(+)ECs). We quantified spontaneous beating rates, synchrony, and coordination between different cardiomyocyte clusters using confocal imaging of Fura Red-detected calcium transients and computer-assisted image analysis. RESULTS After 8 days in culture, 94% ± 6% of the NKX2-5GFP(+) cells were beating when hESCs embryonic bodies were plated on E4(+)ECs compared with 34% ± 12.9% for controls consisting of hESCs cultured on BD Matrigel (BD Biosciences) without ECs at Day 11 in culture. The spatial organization of beating areas in cocultures was different. The GFP(+) cardiomyocytes were close to the E4(+)ECs. The average beats/min of the cardiomyocytes in coculture was faster and closer to physiologic heart rates compared with controls (50 ± 14 [n = 13] vs 25 ± 9 [n = 8]; p < 0.05). The coculture with ECs led to synchronized beating relying on the endothelial network, as illustrated by the loss of synchronization upon the disruption of endothelial bridges. CONCLUSIONS The coculturing of differentiating cardiomyocytes with Akt-activated ECs but not EC-conditioned media results in (1) improved efficiency of the cardiomyocyte differentiation protocol and (2) increased maturity leading to better intercellular coupling with improved chronotropy and synchrony.

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