Course Overview
STEMCELL Technologies
1618 Station Street
Vancouver, CA V6A 1B6
New England Cord Blood Bank
500 Donald Lynch Blvd
Marlborough, MA
01752 United States
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park,
Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Experimental Transfusion Medicine Dresden
c/o DRK Blutspendedienst Nord-Ost gGmbH
Blasewitzer Str. 68/70
01307 Dresden, Germany
Gain hands-on experience in the set-up, identification, and enumeration of hematopoietic progenitor cells using MethoCult™, the gold standard media for hematopoietic colony-forming unit (CFU) assays. Participants working with human cord blood, bone marrow, or mobilized peripheral blood samples will gain expertise in the enumeration of hematopoetic colonies through comparative counting exercises with the instructors. The STEMvision™ instrument designed specifically for the imaging and automated counting of colonies in CFU assays will be introduced.
Participants Will Receive
- Course booklet and electronic versions of instructional material
- Tote bag with sample-size products (where applicable)
- Certificate following successful completion of the course
- Lunch and refreshments on each day and dinner hosted on one of the evenings
Speakers
Speakers will vary depending on course topic, date, and location.

STEMCELL Technologies

STEMCELL Technologies

STEMCELL Technologies
Course Agenda (Tentative)
Note that each day will end at approximately 4:30 pm and the course dinner taking place on one of the evenings.
Day One
-
Welcome Presentation and Introductions
-
Lecture: Introduction to Hematopoiesis and the Colony-Forming Unit (CFU) Assay
-
Lecture 2: CFU Assay Set Up and Colony Identification
-
Practical Session 1 : CFU Assay Set Up Procedure
- Incubator maintenance
- Sample processing with ErythroClear™ Red Blood Cell Depletion Kit
- CFU assay setup
- Demo sample thawing and cell counting
-
Practical Session 2: Manual Colony Identification
-
Practical Session 3: Comparative Colony Counting
-
Wrap Up and Q&A
Day Two
-
Lecture: Standardization Tools for the Hematopoietic CFU Assay
-
Practical Session: Review of Manual Colony Identification Criteria and Comparative Counting
-
Practical Session: Review and Analysis of Group Comparative Counts
-
Lecture: Introduction to Automated and Standardized Colony Counting for the CFU Assay with STEMvision™
-
Practical Session: Operations of STEMvision™Wrap up Session:
- Q&A
- Quiz
- Course Evaluation
- Certificate Presentation
Accommodations
Vancouver, CA
Boston, US
Cambridge, UK
Dresden, DE
Contact education@stemcell.com for information on special rates (when applicable).
Policy
Travel Visas
Travel Visa Requirements
CanadaUnited States
United Kingdom
Germany