Standardization of the Hematopoietic Progenitor Assay Training Course

Two-day hands-on training on the CFU Assay for human cord blood and bone marrow samples

More Views

Standardization of the Hematopoietic Progenitor Assay Training Course

Course will be held on March 7-8, 2018 in Vancouver, Canada

Registration/Cancellation Deadline 02/06/2018
Catalog #00215
1,499 USD

Cancel

Standardization of the Hematopoietic Progenitor Assay Training Course

​Course will be held on March 15-16, 2018 in Dresden, Germany. This session will feature a guest lecture by Dr. Torsten Tonn

Registration/Cancellation Deadline 02/13/2018
Catalog #00215GER
1,499 USD

Cancel

Standardization of the Hematopoietic Progenitor Assay Training Course

​Course will be held on May 15-16, 2018 in Cambridge, United Kingdom

Registration/Cancellation Deadline 04/14/2018
Catalog #00215UK
1,499 USD

Cancel

Course Overview

Course Detail

Training and Education
Training Programs
Cord Blood Banking; Stem Cell Biology; Transplantation
Hematopoietic Stem and Progenitor Cells
Human
Two Days
STEMCELL Technologies
1618 Station Street
Vancouver, BC
V6A 1B6 Canada
STEMCELL Technologies UK Ltd.
Building 7100, Cambridge Research Park, Beach Drive, Waterbeach
Cambridge, UK, CB25 9TL
Experimental Transfusion Medicine Dresden
c/o DRK Blutspendedienst Nord-Ost gGmbH
Blasewitzer Str. 68/70
01307 Dresden, Germany

This two-day course focuses on human colony forming cells from hematopoietic samples. Gain hands-on experience in the set-up, identification and enumeration of hematopoietic progenitor cells using MethoCult™, the gold standard media formulation for hematopoietic colony-forming unit (CFU) assays. Receive personalized instruction on how to standardize the CFU assay and increase your proficiency and confidence in the CFU assay set up and colony identification. Participants will be introduced to STEMvision™, an instrument designed for imaging and automated counting of CFU colonies from various hematopoietic samples.

What Customers Will Receive

  • Course booklet and electronic versions of instructional material
  • Dinner with STEMCELL Technologies' experts on one of the evenings
  • Lunch and refreshments
  • Certificate upon successful completion of the training course
  • Overview of hematopoiesis and introduction to colony identification
  • Procedures for processing CB samples for CFU analysis
  • Overview of tools for CFU assay standardization
  • Detection and enumeration of progenitors in CB and BM for manual and automated systems
  • Set up the CFU assay using MethoCult™
  • Identify and enumerate hematopoietic colonies derived from CB and BM samples
  • Process CB and BM samples for the CFU assay
  • Participate in comparative counting exercise with instructors and course participants
  • Compare automated counting of CFU colonies from human hematopoietic progenitor cells with STEMvision™

Speakers

Guest Speaker - Dresden, Germany

Torsten Tonn, MD
Chair of Transfusion Medicine at the Medical Faculty Carl Gustav Carus
Technical University Dresden

Prof. Dr. Torsten Tonn is the chair of Transfusion Medicine at the Medical Faculty Carl Gustav Carus Dresden. Prof. Tonn´s research focuses on facilitating cell-based therapies into clinical practice. He has more than 20 years of experience in the procurement, harvest and release testing of hematopoietic stem cells (HSCs). In addition, he pioneered the GMP compliant manufacturing and release testing of bone marrow stem cells for myocardial infarction, Streptamer-isolated CMV specific T cells for recurrent viremia post stem cell transplantation, and the first clinical trials using the natural killer cell line NK-92. Potency and release testing are crucial for safe and efficient cell therapies. For his lecture, Prof. Tonn will be speaking about the role of CFU assays as release criteria for HSCs grafts in blood stem cell transplantation.

.

Raymond Lam
Manager, Scientific Knowledge
STEMCELL Technologies

Raymond Lam is the Manager of Scientific Knowledge, Europe at STEMCELL Technologies. Raymond joined STEMCELL in 2004 as a Quality Control Analyst, where he was responsible for testing of all finished products. He then transferred to the Product and Scientific Support department to directly respond to customer inquiries on protocol and products. In 2008, Raymond became a Field Applications Specialist, supporting customers in the field as well as conducting training and seminars at academic institutions. Within his current role, Raymond oversees the expansion and standardization of educational training programs globally for staff, customers, and distributors on STEMCELL Technologies’ products and protocols.


Katharina Debowski, PhD
Field Applications Scientist
STEMCELL Technologies

Dr. Katharina Debowski is a Field Applications Scientist for hematopoietic stem and progenitor cell (HSPC) biology at STEMCELL Technologies. She completed her studies in Germany where she obtained a Ph.D. in biochemistry and did her postdoctoral research at Research Center Jülich. Prior to joining STEMCELL Technologies, Dr. Debowski was a Scientist at the German Primate Center with the Stem Cell Biology Unit where her focus lied in the establishment, characterization, and genetic manipulation of non-human primate stem cells. In her current role, Dr. Debowski is responsible for delivering seminars within Europe and providing technical support in the field relating to STEMCELL’s HSPC product portfolio.

Course Agenda (Tentative)

Day One

  • Welcome Presentation and Introductions
  • Lecture 1: Introduction to Hematopoiesis and the Colony Forming Unit (CFU) Assay
  • Lecture 2: CFU Assay Setup and Colony Identification
  • Practical Session 1 : CFU Assay Setup Procedure
    • Incubator maintenance
    • Sample processing with ErythroClear™ Red Blood Cell Depletion Kit
    • Cell counting
    • CFU assay setup
  • Practical Session 2: Manual Colony Identification for Human Samples
  • Practical Session 3: Comparative Colony Counting
  • Wrap Up and Q&A
  • Dinner with Course Trainers (Courtesy of STEMCELL Technologies)

Day Two

  • Guest Lecture by Prof. Dr. Torsten Tonn from Technical University Dresden (Dresden, Germany location only)
  • Lecture 3: Overview of Tools for CFU Assay Standardization and Insight on the CFU Assay from Global Proficiency Testing Programs
  • Practical Session 4: Review of Manual Colony Identification Criteria and Comparative Counting
  • Practical Session 5: Review and Analysis of Group Comparative Counts
  • Lecture 4: Introduction to Automated and Standardized Colony Counting for the CFU Assay with STEMvision™
  • Practical Session 6: Operations of STEMvision™
  • Wrap up Session:
    • Discussion
    • Quiz
    • Evaluation
    • Certificate Presentation

Accommodations

Dresden, DE

Rothenburger-Hof
Rothenburger-Str. 15-17, 01009, Dresden, Saxony, Germany
Holiday Inn Express Dresden City Centre
Dr-Kuelz Ring 15A, 01067, Dresden, Saxony, Germany
ibis Budget Dresden City
Wilsdruffer Str 25, 01067, Dresden, Saxony, Germany

Vancouver, CA

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Sheraton Wall Centre
1088 Burrard St. Vancouver, BC V6Z2R9
Tel: 1-604-331-1000
Hampton Inn & Suites by Hilton
111 Robson St, Vancouver, V6B 2A8
Tel: 1-877-602-1008 or 1-604-602-1008 or 1-604-605-1465
Executive Hotel LeSoleil
567 Hornby St, Vancouver, BC V6C 2E8
Tel: 1-604 632-3000

Cambridge, UK

Holiday Inn Cambridge
Lakeview, Bridge Rd, Impington, Cambridge CB24 9PH, UK
Five minute taxi ride to course location.

Please contact education@stemcell.com for more information on special seasonal rates (when applicable).

Policy

Travel Visas

Citizens may require a visa in order to enter the country as visitors. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that travel visas may take up to 30 days to process.

Canada Travel Visa Requirements
United Kingdom Travel Visa Requirements
Germany Travel Visa Requirements

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the position.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the course date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Scientific Resources

Educational Materials

(4)
Chat with an Expert