RoboSep™-C Human T Cell Isolation Kit
Immunomagnetic negative selection kit
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RoboSep™-C Human T Cell Isolation Kit -
Label for RoboSep™-C Human T Cell Isolation Kit
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Required Products
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Automated closed-system immunomagnetic cell isolation instrument -
For automated cell isolation with RoboSep™-C -
Cell separation buffer -
Compatible antibodies for purity assessment of isolated cells
Overview
The RoboSep™-C Human T Cell Isolation Kit is designed to isolate untouched and highly purified T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.
This kit targets non-T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted
cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and
concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow
cytometry, culture, or cryopreservation.
This kit targets non-T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted
cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and
concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow
cytometry, culture, or cryopreservation.
Advantages:
⦁ Fully automated large-scale isolation in a closed system
⦁ Up to 99% purity with high recovery
⦁ Untouched, viable cells
⦁ Up to 99% purity with high recovery
⦁ Untouched, viable cells
Components:
- RoboSep™-C Human T Cell Isolation Cocktail, 10 mL
- RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Magnet Compatibility:
• RoboSep™-C (Catalog #100-0186)
Subtype:
Cell Isolation Kits
Cell Type:
T Cells
Species:
Human
Sample Source:
Leukapheresis
Selection Method:
Negative
Application:
Cell Isolation
Brand:
RoboSep
Area of Interest:
Cell Therapy; Drug Discovery and Toxicity Testing; Immunology
Related Products
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Human Peripheral Blood Leukopak, Fresh
Primary human cells, fresh -
RoboSep™-C Human CD4+ T Cell Isolation Kit
Immunomagnetic negative selection kit -
RoboSep™-C Human CD8+ T Cell Isolation Kit
Immunomagnetic negative selection kit
Labeling Antibodies
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Anti-Human CD4 Antibody, Clone OKT4
Mouse monoclonal IgG2b antibody against human, rhesus, cynomolgus CD4 -
Anti-Human CD8a Antibody, Clone RPA-T8
Mouse monoclonal IgG1 antibody against human, rhesus, cynomolgus CD8a -
Anti-Human CD3 Antibody, Clone UCHT1
Mouse (BALB/c) monoclonal IgG1 antibody against human, chimpanzee CD3
Scientific Resources
Product Documentation
Document Type
Product Name
Catalog #
Lot #
Language
Educational Materials
(5)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data
Figure 1. Typical RoboSep™-C Human T Cell Isolation Profile
Starting from a fresh human peripheral blood leukopak, the T cell content (CD3+) of the isolated fraction is typically 96.0 ± 3.2% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 65.9% and 97.5%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.