RoboSep™-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

RoboSep™-C Human CD8+ T Cell Isolation Kit

Immunomagnetic negative selection kit

RoboSep™-C Human CD8+ T Cell Isolation Kit
1 x 10^10 cells
Catalog # 100-0202

Immunomagnetic negative isolation of untouched CD8+ T cells from leukopaks

Product Advantages


  • Fully automated large-scale isolation in a closed system

  • Up to 94% purity with high recovery

  • Untouched, viable cells

What's Included

  • RoboSep™-C Human CD8+ T Cell Isolation Cocktail, 10 mL
  • RoboSep™-C Dextran RapidSpheres™ 30106, 10 mL
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.

Overview

The RoboSep™-C Human CD8+ T Cell Isolation Kit is designed to isolate untouched and highly purified CD8+ T cells from fresh human peripheral blood leukopaks using RoboSep™-C (Catalog #100-0185 and #100-0186) for automated immunomagnetic negative selection.

This kit targets non-CD8+ T cells for removal with antibodies recognizing specific cell surface markers. Using RoboSep™-C in a fully automated protocol, unwanted cells are labeled with antibodies and magnetic particles, and separated without columns using a RoboSep™-C magnet. The desired untouched cells are washed and concentrated by RoboSep™-C and transferred to a final product bag. Isolated cells are immediately available for downstream applications such as flow cytometry, culture, or cryopreservation.
Magnet Compatibility
• RoboSep™-C (Catalog #100-0185)
• RoboSep™-C (Catalog #100-0186)
Subtype
Cell Isolation Kits
Cell Type
T Cells, T Cells, CD8+
Species
Human
Sample Source
Leukapheresis
Selection Method
Negative
Application
Cell Isolation
Brand
RoboSep
Area of Interest
Cell Therapy Research, Drug Discovery and Toxicity Testing, Immunology

Data Figures

FACS Profile Results with RoboSep™-C Human CD8+ T Cell Isolation Kit

Figure 1. Typical RoboSep™-C Human CD8+ T Cell Isolation Profile

Starting with a fresh human peripheral blood leukopak, the CD8+ T cell content (CD3+CD8+) of the isolated fraction is typically 86.8 ± 5.4% (mean ± SD). In the above example, the purities of the start and final isolated fractions are 16.5% and 93.3%, respectively.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
100-0202
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
100-0202
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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