Rat Recombinant IFN-gamma

Interferon-gamma

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Interferon-gamma
From: 26 USD

Overview

Interferon-gamma (IFN-γ) is the only member of the type II class of interferons. It binds to a heterodimeric receptor complex, consisting of IFN-γ receptors 1 and 2, and initiates signal transduction via the JAK/STAT pathway, which culminates in the transcription and activation of many genes that control a diverse array of immunological functions (De Weerd & Nguyen; Krause et al.). IFN-γ is produced by T and NK cells, and in smaller amounts by dendritic cells and macrophages. IFN-γ release assays, which rely on T cells releasing IFN-γ when exposed to specific antigens, are used in diagnosis of diseases including tuberculosis. IFN-γ is controlled by cytokines secreted in response to infection, such as interleukin (IL) 12 and IL-18 (Schroder). IFN-γ stimulates the antimicrobial and antitumor activity of macrophages, NK cells, and neutrophils (Billiau & Matthys) by promoting the activation of microbial effector functions including production of reactive oxygen species, NO intermediates, and complement (Schroder). IFN-γ enhances MHC class I and II expression in dendritic cells and mononuclear phagocytes, as well as the production of IL-12 by dendritic cells (Schroder).
Alternative Names:
Immune interferon, Interferon-γ, MAF, Type II interferon, T cell interferon
Cell Type:
B Cells; T Cells; Monocytes
Species:
Rat
Area of Interest:
Immunology; Cancer Research
Molecular Weight:
15.4 kDa
Purity:
≥ 95%

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Educational Materials

(1)

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Data and Publications

Data

(A) The biological activity of Rat Recombinant IFN-gamma was tested by its ability to inhibit cell growth of WEHI-279 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is less than 0.5 ng/mL. (B) 2 μg of Rat Recombinant IFN-gamma was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining.

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