Overview
This antibody clone has been verified for use as an isotype control antibody for assessing non-specific binding to cells in applications such as flow cytometry and immunofluorescence microscopy applications (surface and intracellular staining).
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(2)Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
Data

Figure 1. Data for Unconjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, followed by a mouse anti-rat IgG2b antibody, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Catalog #60030) followed by a mouse anti-rat IgG2b antibody, FITC.

Figure 2. Data for Alexa Fluor® 488-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Alexa Fluor® 488 (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Alexa Fluor® 488, Catalog #60030AD).

Figure 3. Data for APC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, APC, Catalog #60030AZ).

Figure 4. Data for Biotin-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, Biotin followed by streptavidin (SAV) APC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, Biotin, Catalog #60030BT) followed by SAV APC.

Figure 5. Data for FITC-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, FITC (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, FITC, Catalog #60030FI).

Figure 6. Data for PE-Conjugated
Flow cytometry analysis of C57BL/6 mouse splenocytes labeled with Rat IgG2b, kappa Isotype Control Antibody, Clone RTK4530, PE (solid line histogram). Filled histogram shows labeling with a rat IgG2b, kappa positive control antibody (Anti-Mouse CD45 Antibody, Clone 30-F11, PE, Catalog #60030PE).