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Reversion of Primed hPSCs to Naive-Like State Training Course

Two-day hands-on training to support human pluripotent stem cell research

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Reversion of Primed hPSCs to Naïve-Like State Training Course

Course will be held in Vancouver, Canada.
Complete the Waitlist Form to be notified when the next session is available.

Catalog #00226
1,499 USD

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Course Overview

Course Detail

Training and Education
Training Programs
Stem Cell Biology; Drug Discovery and Toxicity Testing
Pluripotent Stem Cells
Human
Two Days
STEMCELL Technologies
1618 Station Street
Vancouver, BC
V6A 1B6 Canada

This two­-day in-person course covers the maintenance of human pluripotent stem cells (hPSCs) in a feeder-free culture system and the reversion of primed state hPSCs to naïve-like state using a defined media developed under license from the Weizmann Institute of Science. Methods to differentiate hPSCs towards different lineages will also be covered. Over half of the course will be devoted to hands-­on laboratory techniques led by expert scientists and educators from STEMCELL Technologies. Personalized instruction will provide participants with a thorough understanding of how to maintain and characterize hPSCs in primed and naïve-like states.

Participants Receive

  • Course booklet and electronic versions of instructional material
  • Dinner with STEMCELL Technologies' experts on the first evening
  • Lunch and refreshments
  • Certificate upon successful completion of the course
  • Culture and characterization of primed and naïve-like hPSCs
  • Experimental optimization and troubleshooting
  • Common downstream applications
  • Maintain hPSCs in mTeSR™1 and Matrigel™ or TeSR™-E8™ and Vitronectin-XF™
  • Prepare mouse embryonic fibroblasts (MEFs) for reverting hPSCs to naïve-like state
  • Maintain naïve-like hPSCs in RSeT™
  • Monitor the progression of primed hPSCs to naïve-like state
  • Form uniformly sized embryoid bodies (EBs) using AggreWell™

Speakers

Dr. Arwen Hunter
Senior Scientist, Research and Development
STEMCELL Technologies

Dr. Arwen Hunter is a senior scientist of the Pluripotent Stem Cell Group in Research and Development at STEMCELL Technologies. Arwen received her PhD from the University of British Columbia where she focused on endothelial ischemic injury and cardiac allograft vasculopathy. She then performed postdoctoral training at Stanford University where she generated patient specific induced pluripotent stem cells (iPSCs) and iPSC-derived endothelial cells to model vascular disease. In addition, she examined novel strategies for nuclear reprogramming.

Dr. Melanie Kardel
Senior Scientist, Research and Development
STEMCELL Technologies

Dr. Melanie Kardel is a senior scientist of the Pluripotent Stem Cell Group in Research and Development at STEMCELL Technologies. Melanie has a background in genetics and cell biology with numerous years of experience in working with hPSCs. She obtained her PhD from the University of British Columbia working with hPSCs and their differentiation into hematopoietic cells. She joined STEMCELL Technologies in 2012 and her current research areas include the development of technologies related to hPSC maintenance, characterization, and differentiation.

Course Agenda (Tentative)

Day One

ES & iPS Cell Maintenance and Embryoid Body Formation

  • 9:00 - 10:30
    Lecture 1: Feeder-Free Culture Systems for Human ES and iPS Cells
  • 10:30 - 10:45
    Break
  • 10:45 - 12:00
    Practical Session 1: Evaluate Quality of hES & iPS Cell Cultures and Practice Plate Coating
    • Review time course of iPSC cultures to evaluate colony morphology
    • Practice plate coating with mTeSR™1 & Matrigel© or TeSR™-E8™ & Vitronectin XF™
  • 12:00 - 1:00
    Lunch (Courtesy of STEMCELL Technologies)
  • 1:00 - 2:30
    Practical Session 2: Passaging and Long-Term Maintenance of hES & iPS Cells
    • Hands-on practice of enzyme-free passaging of hES & iPS cells using Gentle Cell Dissociation Reagent and ReLeSR™
  • 2:30 - 3:15
    Practical Session 3: Cryopreservation and Thawing of hES & iPS Cells
    • Prepare single cell suspension
    • Prepare uniformly sized EBs using AggreWell™ plates
    • Demo of cryopreservation and thawing of clump passaged hPSCs using CryoStor© CS10
    • Demo of cryopreservation and thawing of single cell hPSCs using FreSR™-S
  • 3:15 - 3:30
    Break
  • 3:30 - 4:30
    Lecture 2: Differentiating Human ES and iPS Cells in Defined Culture Environments with STEMdiff™ Kits
  • 4:30 - 4:45
    Wrap Up and Q&A Session
  • 5:30 - 7:30
    Dinner with Course Trainers

Day Two

Reversion and Maintenance of Primed hPSCs to Naïve-Like State

  • 9:00 - 10:30
    Lecture 3: Overview of hPSC Naïve-Like State. Reversion and Maintenance of Primed hPSCs to Naïve-Like State
  • 10:30 - 10:45
    Break
  • 10:45 - 11:45
    Practical Session 4: Preparation of Irradiated Mouse Embryonic Fibroblasts (iMEFs) for Reverting Primed hPSCs to Naïve-Like State
  • 11:45 - 12:45
    Lunch (Courtesy of STEMCELL Technologies)
  • 12:45 - 2:15
    Practical Session 5: Reversion of Primed hPSCs to Naïve-Like State
    • Plate primed hPSCs as aggregates in mTeSR™1 onto iMEFs
    • Evaluate quality of newly reverted naïve-like hPSCs
  • 2:15 - 2:30
    Break
  • 2:30 - 3:45
    Practical Session 6: Maintenance of Established Naïve-Like hPSCs
    • Passage established naïve-like hPSCs
    • Evaluate the quality of fully reverted naïve-like hPSCs
  • 3:45 - 4:30
    Lecture 4: Characterization and Downstream Differentiation of Naïve-Like hPSCs
  • 4:30 - 5:00
    Course Wrap Up
    • Q&A Session
    • Quiz
    • Evaluation
    • Certificate Presentation

Accommodations

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel: 1-877-602-1008 or 1-604-602-1008 or 1-604-605-1465
Sheraton Wall Centre
1088 Burrard St. Vancouver, BC V6Z2R9
Tel: 1-604-331-1000
Hampton Inn & Suites by Hilton
111 Robson St, Vancouver, V6B 2A8
Tel: 1-877-602-1008 or 1-604-602-1008 or 1-604-605-1465
Executive Hotel LeSoleil
567 Hornby St, Vancouver, BC V6C 2E8
Tel: 1-604 632-3000

Please contact education@stemcell.com for more information on special seasonal rates (when applicable).

Policy

Travel Visa

Non-Canadian and American citizens may require visitor visas to enter Canada. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that Canadian travel visas may take up to 30 days to process.

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the position.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the course date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Scientific Resources

Educational Materials

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