Intestinal Organoid Training

Online and in-person training courses to support the culture of intestinal organoids

Intestinal Organoid Virtual Training Starter Kit

Starter Kit to complement on-demand training course. Access the On-Demand Intestinal Organoid Course through the blue box below

Catalog #00261

Intestinal Organoid In-Person Training

Next session dates will be based on participant demand and availability

Catalog #00262
Already have the Intestinal Organoid Starter Kit? Access the On-Demand Intestinal Organoid Course here.
Required Products
  1. Gentle Cell Dissociation Reagent
    Gentle Cell Dissociation Reagent

    cGMP, enzyme-free cell dissociation reagent

  2. DMEM/F-12 with 15 mM HEPES
    DMEM/F-12 with 15 mM HEPES

    Dulbecco's Modified Eagle's Medium/Nutrient Ham's Mixture F-12 (DMEM/F-12) with 15 mM HEPES buffer

  3. D-PBS Without Ca++ and Mg++
    D-PBS (Without Ca++ and Mg++)

    Dulbecco’s phosphate-buffered saline without calcium and magnesium

Overview

Virtual Training (Catalog #00261)

Learn to culture mouse intestinal organoids in a defined and serum-free medium from the convenience of your own lab. Gain access to lectures and step-by-step procedural videos where our scientists demonstrate the entire protocol for growing mouse intestinal organoids.


Participants Receive


    In-Person Training (Catalog #00262)

    This course provides a comprehensive overview of the mouse intestinal organoid culture workflow. Learn to establish cultures from mouse small intestine and colon and how to maintain them in IntestiCult™ Organoid Growth Medium (Mouse). Participants will also gain experience with performing downstream assays.


    Participants Receive

    • Course booklet and electronic versions of instructional material
    • Certificate following successful completion of the course
    • Lunch and refreshments on each day and dinner hosted on one of the evenings
    Training and Education
    Training Programs
    Stem Cell Biology; Drug Discovery and Toxicity Testing; Epithelial Cell Biology
    Intestinal Cells
    Mouse
    Online (Catalog #00261)

    Canada:
    STEMCELL Technologies
    1618 Station Street
    Vancouver, CA V6A 1B6

    United Kingdom:
    STEMCELL Technologies UK Ltd.
    Building 7100, Cambridge Research Park,
    Beach Drive, Waterbeach
    Cambridge, UK, CB25 9TL

    Speakers

    Speakers will vary depending on location.


    Dr. Ryan Conder
    Ryan Conder, PhD
    Senior Scientist and Team Lead of the Intestinal Group
    STEMCELL Technologies
    Dr. Salvatore Simmini
    Salvatore Simmini, PhD
    Scientist, Intestinal
    STEMCELL Technologies

    Agenda

    Virtual Training (Catalog #00261)

    • Lecture 1: Introduction to Intestinal Organoid Culture
    • Lecture 2: Functional Applications of Intestinal Organoid Culture
    • Session 1:
      • Thaw cryopreserved intestinal organoids and culture in IntestiCult™ Organoid Growth Medium (Mouse)
      • Observe organoid morphology in culture after 3 hours, 1 day and 2 days
    • Session 2: Observe time course of organoid morphology in culture and discuss long-term maintenance
    • Session 3:
      • Evaluate optimal organoid morphology for passaging and cryopreservation.
      • Passage mouse intestinal organoids and discuss cryopreservation
    • Session 4: Observe organoid morphology one day after passaging

    In-Person Training (Catalog #00262)

    Day One

    • Welcome Presentation and Introductions
    • Lecture: Overview of Intestinal Organoid Culture
    • Practical Session: Mouse Intestinal Organoid Morphology Cultured in IntestiCult™ Organoid Growth Medium.
      • Observe time course of organoid morphology
      • Demonstration and hands-on practice with passaging mouse intestinal organoids
    • Practical Session: Mouse Crypt Isolation and Establishment of Organoid Culture from Small Intestine and Colon
    • Wrap Up and Q&A

    Day Two

    • Lecture: Applications of Mouse Intestinal Organoids and Current Development
    • Practical Session: Mouse Intestinal Organoid Maintenance
      • Observe organoid cultures from day 1
      • Demonstration of cryopreservation and thawing of mouse intestinal organoids
    • Practical Session: Downstream Assays for Intestinal Organoids
    • Wrap Up:
      • Q&A
      • Quiz
      • Course Evaluation
      • Certificate Presentation

    Scientific Resources

    Product Documentation

    Document Type Product Name Catalog # Lot # Language
    Document Type
    Product Information Sheet 1
    Product Name
    Intestinal Organoid Virtual Training Starter Kit
    Catalog #
    00261
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 2
    Product Name
    Intestinal Organoid Virtual Training Starter Kit
    Catalog #
    00261
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 1
    Product Name
    Intestinal Organoid In-Person Training
    Catalog #
    00262
    Lot #
    All
    Language
    English
    Document Type
    Product Information Sheet 2
    Product Name
    Intestinal Organoid In-Person Training
    Catalog #
    00262
    Lot #
    All
    Language
    English

    Educational Materials (5)

    Brochure
    2019-2020 Cell Culture Training Catalog
    Video
    How to Count Intestinal Crypts for Mouse Organoid Cultures
    1:09
    How to Count Intestinal Crypts for Mouse Organoid Cultures
    Video
    STEMCELL Technologies Inc. Training Programs
    2:13
    STEMCELL Technologies Inc. Training Programs
    Webinar
    Modeling Host-Microbe Interactions Using Human Intestinal Organoids
    16:31
    Modeling Host-Microbe Interactions Using Human Intestinal Organoids
    Webinar
    GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
    24:56
    GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation

    Product Applications

    This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

    Research Area
    Workflow Stages

    Related Products

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