Human Intestinal Organoid Maintenance & Downstream Applications
Learn to maintain high-quality human intestinal organoids and about their downstream applications through live virtual training.
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Overview
Human intestinal organoids provide researchers with a physiologically relevant in vitro tool for studying intestinal cell biology and modeling human diseases. Get started with the protocols and best practices for culturing human intestinal organoids in your own lab by registering for this three-day, part-time course consisting of live-streamed, interactive lectures and laboratory demonstrations. Guided by STEMCELL Technologies’ in-house organoid experts, you will gain the skills required to successfully maintain high-quality human intestinal organoids and learn about their downstream applications. You will also have the opportunity to share ideas with fellow participants in the Q&A and wrap-up sessions.
Learning Outcomes:
- Understand the historical context of human intestinal organoids.
- Describe the cellular and molecular processes driving intestinal stem cell function.
- Discern the pros and cons of organoid culture compared to 2D culture and animal models.
- Understand the workflow and critical steps for establishing intestinal organoids from human intestinal biopsies.
- Assess the quality of human intestinal organoids in culture.
- Know how to quantify the culture expansion of human intestinal organoids.
- Describe the cellular composition of differentiated organoids and their applications.
- Understand how to culture intestinal Wnt-independent cancer organoids.
- Describe the workflows for intestinal monolayers and apical-out organoids.
As part of the course, you will receive:
- An electronic copy of the course book
- Certificates of course completion from STEMCELL Technologies and Continuing Professional Development (CPD), UK
- Post-training support from the trainers and STEMCELL’s Scientific Support team
Space is limited, please apply early for the best chance of securing a seat for your desired date. Registration closes one week before the course date.
Agenda
Day 1 (4.25 hrs, including introduction, break, and wrap-up/Q&A)
- Lecture 1: Introduction to Intestinal Organoid Culture Systems (Human) (1 hr)
- Lecture 2: Establishing Human Intestinal Organoids from Primary Tissue (1 hr)
- Guest Lecture: Dissecting host-microbiota interactions in disease using organoids. MSc Cayetano Pleguezuelos, Hubrecht Institute. (1 hr)
Day 2 (4.25 hrs min, including break and wrap-up/Q&A)
- Lecture 3: Maintaining and Assessing High-Quality Human Intestinal Organoid Cultures (1 hr)
- Lab Live-Stream: Maintenance and Passaging of Human Intestinal Organoids (2.25 hrs)
Day 3 (3 hrs, including break and wrap-up)
- Lecture 4: Organoid Applications (1 hr)
- Differentiation of Human Intestinal Organoids
- 3D to the 2D culture system
- Polarity Inversion of Human Intestinal Organoids
- Cancer samples
- Lab Live-Stream: Downstream Assays for Intestinal Organoids (3 hrs)
- Single-cell preparation, 2D culture generation
- PFA fixation
STEMCELL Technologies is approved as a provider of continuing education programs in the clinical laboratory sciences by The American Society for Clinical Laboratory Science (ASCLS) Professional Acknowledgment for Continuing Education (P.A.C.E.®) Program.
This course is certified by the United Kingdom CPD Certification Service, which may be applicable to individuals who are members of, or are associated with, UK-based professional bodies. This course consists of approximately 10.25 hours of learning.
Organisme de formation enregistré sous le n° 82 38 02893 38 depuis 2001.
Note: STEMCELL Technologies accept no responsibility, and cannot be held responsible, for the claiming or validation of hours or points.
Policy
Payment information is required to complete course registration; you will not be charged until your registration is confirmed. Registration and acceptance into the course is subject to review by STEMCELL Technologies. You will be notified within one week of submission on your status.
If you are eligible for the course and registration for your desired date is full, you will be added to our next available session. Space is limited to ensure personalized instruction. Please apply early to ensure you save your seat.
Please be advised that STEMCELL does not permit the recording of courses, technical support consultations, or product demonstrations delivered by STEMCELL Technologies, whether conducted in person, over video conference, or by phone. This policy applies to all audiences, including but not limited to customers, course attendees, and distributors. All training content is copyrighted by STEMCELL Technologies and violations of this policy will be pursued under copyright law.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
