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hPSC Training Course: Reprogramming, Maintenance and Reversion to Naïve-Like State

Three-day hands-on training to support human pluripotent stem cell research

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hPSC Training Course: Reprogramming, Maintenance and Reversion to Naïve-Like State

Course will be held in Vancouver, Canada. Be the first to know about the next available session.

Catalog #00227
2,250 USD

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Course Overview

Course Detail

Training and Education
Training Programs
Stem Cell Biology; Drug Discovery and Toxicity Testing
Pluripotent Stem Cells
Human
Three Days
STEMCELL Technologies
1618 Station Street
Vancouver, BC
V6A 1B6 Canada

This three-day in-person course covers the reprogramming of somatic cells to induced pluripotent stem (iPS) cells, their maintenance in a feeder-free culture system and the reversion of primed state human pluripotent stem cells (hPSCs) to naïve-like state using a defined media developed under license from the Weizmann Institute of Science. Over half of the course will be devoted to hands-­on laboratory techniques led by expert scientists and educators from STEMCELL Technologies. Small class size ensures personalized instructions and hands-on experience.

Participants Receive

  • Course booklet and electronic versions of instructional material
  • Dinner with STEMCELL Technologies' experts on the second evening
  • Lunch and refreshments
  • Certificate upon successful completion of the course
  • Isolation, selection and expansion of blood and fibroblast cells prior to reprogramming
  • Culture and characterization of primed and naïve-like hPSCs
  • Experimental optimization and troubleshooting
  • Common downstream applications
  • Isolate peripheral blood mononuclear cells with SepMate™ and enrich CD34+ cells using EasySep™
  • Observe transfection of somatic cells with ReproRNA™-OKSGM
  • Review morphological changes during reprogramming process using TeSR™-E7™ or ReproTeSR™
  • Identify and select fully reprogrammed iPS cell colonies
  • Maintain hPSCs in mTeSR™1 and Matrigel™ or TeSR™-E8™ and Vitronectin-XF™
  • Form uniformly sized embryoid bodies (EBs) using AggreWell™
  • Prepare mouse embryonic fibroblasts (MEFs) for reverting hPSCs to naïve-like state
  • Maintain naïve-like hPSCs in RSeT™

Speakers

Dr. Arwen Hunter
Senior Scientist, Research and Development
STEMCELL Technologies

Dr. Arwen Hunter is a senior scientist of the Pluripotent Stem Cell Group in Research and Development at STEMCELL Technologies. Arwen received her PhD from the University of British Columbia where she focused on endothelial ischemic injury and cardiac allograft vasculopathy. She then performed postdoctoral training at Stanford University where she generated patient specific induced pluripotent stem cells (iPSCs) and iPSC-derived endothelial cells to model vascular disease. In addition, she examined novel strategies for nuclear reprogramming.

Dr. Melanie Kardel
Senior Scientist, Research and Development
STEMCELL Technologies

Dr. Melanie Kardel is a senior scientist of the Pluripotent Stem Cell Group in Research and Development at STEMCELL Technologies. Melanie has a background in genetics and cell biology with numerous years of experience in working with hPSCs. She obtained her PhD from the University of British Columbia working with hPSCs and their differentiation into hematopoietic cells. She joined STEMCELL Technologies in 2012 and her current research areas include the development of technologies related to hPSC maintenance, characterization, and differentiation.

Course Agenda (Tentative)

Day One

Isolation and Reprogramming of Somatic Cells to iPSCs

  • 9:00 - 9:15
    Welcome and Introductions
  • 9:15 - 10:45
    Lecture 1: Isolation and Reprogramming of Somatic Cells into iPSCs
  • 10:45 - 11:00
    Break
  • 11:00 - 12:30
    Practical Session 1: Isolation of Peripheral Blood Mononuclear Cells
    • Overview of EasySep™ cell separation system
    • Demonstrate preparation of PBMCs using SepMate™
    • Observe culture expanded blood cells using StemSpan™
  • 12:30 - 1:30
    Lunch (Courtesy of STEMCELL Technologies)
  • 1:30 - 3:00
    Practical Session 2: Reprogram Somatic Cells to iPSCs
    • Demonstrate transfection of human fibroblasts to iPSCs using ReproRNA™-OKSGM
    • Observe reprogrammed cells cultured in ReproTeSR™
  • 3:00 - 3:15
    Break
  • 3:15 - 4:30
    Practical Session 3: Hands-on Practice with Selection and Picking of iPSC Colonies
  • 4:30 - 4:45
    Wrap Up and Q&A Session

Day Two

ES & iPS Cell Maintenance and Embryoid Body Formation

  • 9:00 - 10:30
    Lecture 2: Feeder-Free Culture Systems for Human ES and iPS Cells
  • 10:30 - 10:45
    Break
  • 10:45 - 11:45
    Practical Session 4: Evaluate Quality of hPSC Cultures and Practice Plate Coating
    • Review time course of iPSC culture morphology
    • Plate coating with mTeSR™1 & Matrigel© or TeSR™-E8™ & Vitronectin XF™
  • 11:45 - 12:45
    Lunch (Courtesy of STEMCELL Technologies)
  • 12:45 - 2:15
    Practical Session 5: Passaging and Long-Term Maintenance of hES & iPS Cells
    • Hands-on practice of enzyme-free passaging of hES & iPS cells using Gentle Cell Dissociation Reagent and ReLeSR™
  • 2:15 - 2:45
    Practical Session 6: Cryopreservation and Thawing of hES & iPS Cells
    • Demo of cryopreservation and thawing of clump passaged hPSCs using CryoStor© CS10
    • Discussion of cryopreservation and thawing of single cell hPSCs using FreSR™-S
  • 2:45 - 3:45
    Practical Session 7: Generate EBs using AggreWell™
  • 3:45 - 4:00
    Break
  • 4:00 - 5:00
    Lecture 3: Differentiating Human ES and iPS Cells in Defined Culture Environments with STEMdiff™ Kits
  • 5:00 - 5:15
    Wrap Up and Q&A Session
  • 5:30 - 8:00
    Dinner with STEMCELL Technologies

Day Three

Reversion and Maintenance of Primed hPSCs to Naive-Like State

  • 9:00 - 10:30
    Lecture 4: Overview of hPSC Naive-Like State & the Reversion and Maintenance of Primed hPSCs to Naive-Like State
  • 10:30 - 10:45
    Break
  • 10:45 - 11:45
    Practical Session 8: Prepare Mouse Embryonic Fibroblasts (MEFs) for Reverting hPSC Primed to Naive-Like State
  • 11:45 - 12:45
    Lunch (Courtesy of STEMCELL Technologies)
  • 12:45 - 2:15
    Practical Session 9: Maintenance of Established Naïve-Like hPSCs
    • Plate primed hPSCs as aggregates in mTeSR™1 onto iMEFs
    • Evaluate quality of newly reverted naive-like hPSCs
  • 2:15 - 2:30
    Break
  • 2:30 - 3:45
    Practical Session 10: Reversion of Primed hPSCs to Naive-Like State.
    • Passage established naive-like hPSCs
    • Evaluate quality of fully reverted naive-like hPSCs
  • 3:45 - 4:30
    Lecture 5: Characterization and Downstream Differentiation of Naive-Like State Cells
  • 4:30 - 5:00
    Course Wrap Up
    • Q&A Session
    • Quiz
    • Evaluation
    • Certificate Presentation

Accommodations

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Sheraton Wall Centre
1088 Burrard St. Vancouver, BC V6Z2R9
Tel: 1-604-331-1000
Hampton Inn & Suites by Hilton
111 Robson St, Vancouver, V6B 2A8
Tel: 1-877-602-1008 or 1-604-602-1008 or 1-604-605-1465
Executive Hotel LeSoleil
567 Hornby St, Vancouver, BC V6C 2E8
Tel: 1-604 632-3000

Please contact education@stemcell.com for more information on special seasonal rates (when applicable).

Policy

Travel Visa

Non-Canadian and American citizens may require visitor visas to enter Canada. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that Canadian travel visas may take up to 30 days to process.

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the position.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the course date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Scientific Resources

Educational Materials

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