Applications of the Hematopoietic Progenitor Assay Training Course

Three-day hands-on training on the CFU Assay for human, mouse, and rat samples

More Views

Applications of the Hematopoietic Progenitor Assay Training Course

Course will be held on October 11-13, 2017 in Vancouver, Canada

1 Course
Registration/Cancellation Deadline 09/10/2017
Catalog #00217
1,750 USD

Cancel

Course Overview

Course Detail

See Above

Training and Education
Training Programs
Stem Cell Biology; Drug Discovery and Toxicity Testing; Hematologic Malignancies
Hematopoietic Stem and Progenitor Cell
Human, Mouse, Rat and Canine
Three Days
STEMCELL Technologies
1618 Station Street
Vancouver, BC
V6A 1B6 Canada

This three-day course focuses on hematopoietic progenitor assays for human, mouse, rat and canine samples with an emphasis on applying the colony-forming unit (CFU) assay to your research. Participants will gain confidence in performing the CFU assay, identifying and enumerating hematopoietic progenitor cells. The use the CFU assay in drug development and predictive toxicity testing is also discussed. Participants will learn how to effectively design in vitro and in vivo assays to understand the effects of biological pathways on hematopoietic homeostasis and also how to evaluate the effect of compounds on blood and bone marrow (BM) using various mouse models.

Participants Receive

  • Course booklet and electronic versions of instructional material
  • Dinner with STEMCELL Technologies' experts on one of the evenings
  • Lunch and refreshments
  • Certificate upon successful completion of the course
  • Introduction to hematopoiesis and the CFU assay
  • CFU assay set up and colony identification of mouse, human, rat and canine progenitors
  • Applications of the CFU assay and in vitro toxicity testing
  • Review of hematopoietic stem cell in vivo assays
  • Overview of CFU assay to evaluate aspects of hematopoietic biology and action of cytokines
  • Perform CFU assay using MethoCult™
  • Utilize MegaCult™ for the growth and detection of megakaryocyte progenitors(CFU-Mk)
  • Review optimal incubator maintenance
  • Identify CFU colonies derived from blood and BM of mouse, human, rat and canine samples
  • Participate in comparative counting exercises with instructors and participants

Speakers

Dr. Jackie Damen
Scientific Director of the Contract Assay Services
STEMCELL Technologies

Dr. Jackie Damen is the Scientific Director of the Contract Assay Services department at STEMCELL Technologies. In a career spanning over 20 years, Dr. Damen has acquired extensive experience in the field of hematopoiesis, where her key strengths lie in stem cell biology, protein biochemistry and signalling, especially in the area of erythropoietin. She manages all aspects of the Contract Assay Services department which includes client consultation and customized experimental design, execution and analysis. Dr. Damen actively participates in several educational initiatives, regularly providing online talks and presentations of topical interest at high profile conferences such as the Society of Toxicology Annual Meeting. At STEMCELL Technologies, Dr. Damen has been involved in customer training of hematopoietic stem cell biology and colony identification since 2002.

Raymond Lam
Program Manager of Scientific Training
STEMCELL Technologies

Raymond Lam is the Program Manager of Scientific Training in the Education & Product and Scientific Support department at STEMCELL Technologies. He first joined the company as a member of the Quality Control department, where he was responsible for the testing of manufactured products. Raymond subsequently transferred to the Product & Scientific Support Department to directly respond and troubleshoot researcher inquiries. He supported customers in the field with on-site visits and delivered training seminars at academic institutions. In 2011, Raymond transitioned to the Education Department as Senior Instructor, delivering training courses to staff and customers. Within his current role as Program Manager of Scientific Training, Raymond oversees the educational programs for staff and customers, and serves as a lead instructor in several training programs.

Course Agenda (Tentative)

Day One

  • 8:45 - 9:00
    Welcome Presentation & Classroom Introductions
  • 9:00 - 10:00
    Lecture 1: Introduction to Hematopoiesis and the Colony-Forming Unit (CFU) Assay for Human Samples
  • 10:00 - 11:00
    Lecture 2: CFU Assay Set-Up and Colony Identification for Human Samples
  • 11:00 - 11:15
    Break
  • 11:15 - 12:45
    Practical Session 1 : CFU Assay Set-Up Procedure
    • Incubator maintenance
    • Human sample processing with ErythroClear™ Red Blood Cell Depletion Kit
    • Cell counting
    • CFU assay setup
  • 12:45 - 1:30
    Lunch (Courtesy of STEMCELL Technologies)
  • 1:30 - 2:30
    Practical Session 2: Manual Colony Identification for Human Samples
  • 2:30 - 2:45
    Break
  • 2:45 - 4:45
    Practical Session 3: Comparative Colony Counting for Human Samples
  • 4:45 - 5:00
    Wrap Up & Q&A Session
  • 5:00 - 5:15
    Wrap Up Session

Day Two

  • 9:00 - 10:00
    Lecture 3: Introduction to Hematopoiesis and Standardization of the CFU Assay Set-Up for Mouse and Other Species
    Lecture 4: Applications of Hematopoietic CFU Assay for Mouse and Other Species & Overview of CFU-Mks
  • 10:00 - 10:15
    Break
  • 10:15 - 12:15
    Practical Session 4: Review of Manual Colony Identification Criteria and Comparative Counting for Human Samples
  • 12:15 -1:15
    Lunch (Courtesy of STEMCELL Technologies)
  • 1:15 -2:15
    Practical Session 5: Sample Processing and Colony Identification for Mouse Samples
  • 2:15 - 2:30
    Break
  • 2:30 - 3:45
    Practical Session 6: MegaCult™ CFU-Mk Assay
    • Assay Set-Up
    • Colony Identification and Counting
  • 3:45 - 5:00
    Practical Session 7: Colony Identification for Mouse, Rat and Canine Samples
  • 5:00 - 5:15
    Wrap Up and Q&A Session
  • 6:00 - 8:00
    Dinner with Course Trainers

Day Three

  • 9:00 - 10:00
    Lecture 4: Applications of Hematopoietic CFU Assay for Mouse and Other Species & Overview of CFU-Mks
  • 10:00 - 10:15
    Break
  • 10:15 - 11:15
    Lecture 5: Serum-Free Hematopoietic Cell Expansion Supplements and HemaTox™ Kits
  • 11:15 -12:15
    Practical Session 8: Review of Manual Colony Identification Criteria and Comparative Counting for Mouse Samples
  • 12:15 -1:15
    Lunch (Courtesy of STEMCELL Technologies)
  • 1:15 - 2:45
    Practical Session 8 Continued
  • 2:45 - 3:00
    Break
  • 3:00 - 4:30
    Practical Session 9:
    • Review of Manual Colony Identification Criteria & Individual Comparative Counting for Other Species
    • Set up toxicity curve and observe cultures
  • 4:30 - 5:00
    Wrap Up Session
    • Discussion
    • Quiz
    • Evaluation
    • Certificate Presentation

Accommodations

Pan Pacific Vancouver
999 Canada Pl #300, Vancouver, BC, Canada, V6C 3B5
Tel 1-800-663-1515 or 1-604-662-3223
Sheraton Wall Centre
1088 Burrard St. Vancouver, BC V6Z2R9
Tel: 1-604-331-1000
Hampton Inn & Suites by Hilton
111 Robson St, Vancouver, V6B 2A8
Tel: 1-877-602-1008 or 1-604-602-1008 or 1-604-605-1465
Executive Hotel LeSoleil
567 Hornby St, Vancouver, BC V6C 2E8
Tel: 1-604 632-3000

Please contact education@stemcell.com for more information on special seasonal rates (when applicable).

Policy

Travel Visa

Non-Canadian and American citizens may require visitor visas to enter Canada. Please contact us at education@stemcell.com if you require a letter of invitation for your application. Note that Canadian travel visas may take up to 30 days to process.

Cancellation

A 90% refund will be issued if notification is received in writing more than 30 days in advance of the course date. No refund will be given with less than 30 days’ notice unless we are able to find another participant to fill the position.

STEMCELL Technologies reserves the right to cancel the course if we do not receive a minimum number of registrations 30 days in advance of the course date. Should the course be cancelled, your payment will be refunded in full. We recommend that you purchase travel insurance with your flights to cover the possibility of the course being cancelled.

Technical Resources

Educational Materials

(10)
Chat with an Expert