Human Peripheral Blood-Derived M2a Macrophages, SF-Cultured, Frozen

Primary human cells, frozen
Human Peripheral Blood-Derived M2a Macrophages, SF-Cultured, Frozen

Primary human cells, frozen

1.5 x 10^6 cells
Catalog # 200-0374
1,040 USD

Overview

Streamline your assays with ready-to-use, ethically sourced, serum-free-cultured, primary human M2a macrophages. With personalized service, custom products, flexible delivery times, and the option to reserve entire lots to prescreen cells for applications, we help you get the cells you need.

Peripheral blood (PB) monocytes are isolated by negative immunomagnetic selection and cultured for 5 days in serum-free ImmunoCult™-SF Macrophage Medium (Catalog #10961) with macrophage colony-stimulating factor (M-CSF), including 2 days of activation with interleukin 4 (IL-4) to generate M2a macrophages.

Cells are collected using Institutional Review Board (IRB)-approved consent forms and protocols and cryopreserved in animal component-free CryoStor® CS10 medium. Additional documentation and high-resolution HLA typing (Class I and Class II alleles and CMV status) are available upon request. Acid-citrate-dextrose solution A (ACDA) is added during collection as an anticoagulant. Donor specifications (e.g. BMI category, smoking status, ethnicity, etc.) can be requested in the comment box above. Donors are screened for HIV-1, HIV-2, hepatitis B, and hepatitis C.

Certain products are only available in select territories. Please contact your local sales representative or Product & Scientific Support at techsupport@stemcell.com for further information.

Browse our Frequently Asked Questions (FAQs) on Primary Cells.
Contains
CryoStor® CS10
Subtype
Frozen
Cell Type
Macrophages
Species
Human
Cell and Tissue Source
Peripheral Blood
Area of Interest
Cell Therapy, Disease Modeling, Stem Cell Biology
Donor Status
Normal
Purity
≥ 80% CD206+, ≥ 80% CD209+, ≤ 10% CCR7+, and ≤ 10% CD80+ by flow cytometry

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Peripheral Blood-Derived M2a Macrophages, SF-Cultured, Frozen
Catalog #
200-0374
Lot #
All
Language
English

Educational Materials (4)

Brochure
Human Primary Cells
Brochure
Tools For Your Immunology Research
Wallchart
Frequencies of Cell Types in Human Peripheral Blood
Video
How to Thaw Frozen Human Primary Cells
6:05
How to Thaw Frozen Human Primary Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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