Human Peripheral Blood CD19+ B Cells, Frozen

Primary human cells, frozen

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Human Peripheral Blood CD19+ B Cells, Frozen

Primary human cells, frozen

1 x 107 cells

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Catalog #70033
788 USD

Overview

Primary human CD19+ B cells were isolated from peripheral blood (PB) mononuclear cells (MNCs) using positive immunomagnetic separation techniques. PB was collected using one of the following anticoagulants: acid-citrate-dextrose (ACD), acid-citrate-dextrose solution A (ACDA), citrate-phosphate-dextrose (CPD), citrate-phosphate-double-dextrose (CP2D), or citrate-phosphate-dextrose-adenine (CPDA).

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.

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Contains:
• CryoStor® CS10
Subtype:
Frozen
Cell Type:
B Cells
Species:
Human
Cell and Tissue Source:
Peripheral Blood
Donor Status:
Normal
Purity:
The purity of CD19+ B cells is ≥ 90% by flow cytometry.

Scientific Resources

Product Documentation

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Educational Materials

(2)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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Data and Publications

Publications

(1)
British journal of haematology 2018 AUG

Paediatric patients with acute leukaemia and KMT2A (MLL) rearrangement show a distinctive expression pattern of histone deacetylases.

N. Vega-Garc\'ia et al.

Abstract

Histone deacetylase inhibitors (HDACi) had emerged as promising drugs in leukaemia, but their toxicity due to lack of specificity limited their use. Therefore, there is a need to elucidate the role of HDACs in specific settings. The study of HDAC expression in childhood leukaemia could help to choose more specific HDACi for selected candidates in a personalized approach. We analysed HDAC1-11, SIRT1, SIRT7, MEF2C and MEF2D mRNA expression in 211 paediatric patients diagnosed with acute leukaemia. There was a global overexpression of HDACs, while specific HDACs correlated with clinical and biological features, and some even predicted outcome. Thus, some HDAC and MEF2C profiles probably reflected the lineage and the maturation of the blasts and some profiles identified specific oncogenic pathways active in the leukaemic cells. Specifically, we identified a distinctive signature for patients with KMT2A (MLL) rearrangement, with high HDAC9 and MEF2D expression, regardless of age, KMT2A partner and lineage. Moreover, we observed an adverse prognostic value of HDAC9 overexpression, regardless of KMT2A rearrangement. Our results provide useful knowledge on the complex picture of HDAC expression in childhood leukaemia and support the directed use of specific HDACi to selected paediatric patients with acute leukaemia.
STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.