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Human iPSC-Derived Neural Progenitor Cells

Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A

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Human iPSC-Derived Neural Progenitor Cells

Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A

From: 995 USD
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Frozen human neural progenitor cells differentiated from the human induced pluripotent stem cell line, SCTi003-A
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Product Advantages


  • Expand immediately post-thaw with STEMdiff™ Neural Progenitor Medium

  • Save time by starting your differentiation workflow with a highly characterized neural progenitor intermediate

  • Differentiate into forebrain neurons and/or astrocytes using the STEMdiff™ neural system

  • Ensure relevance with neuron-astrocyte co-cultures generated with the same genetic background

  • Obtain high-quality NPCs, derived from the highly characterized control line, SCTi003-A

What's Included

  • Human iPSC-Derived Neural Progenitor Cells (Catalog # 200-0620)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. STEMdiff™ Neural Progenitor Medium
    STEMdiff™ Neural Progenitor Medium

    Medium for maintenance and expansion of neural progenitor cells derived from human ES and iPS cells

  2. STEMdiff™ Neural Progenitor Freezing Medium
    STEMdiff™ Neural Progenitor Freezing Medium

    For cryopreservation of neural progenitor cells generated using STEMdiff™ Neural Induction Medium

  3. STEMdiff™ Forebrain Neuron Differentiation Kit
    STEMdiff™ Forebrain Neuron Differentiation ...

    Differentiation kit for the generation of neuronal precursors from human ES and iPS cell-derived neural progenitor cells

  4. STEMdiff™ Astrocyte Differentiation Kit
    STEMdiff™ Astrocyte Differentiation Kit

    Differentiation kit for the generation of astrocyte precursors from human ES and iPS cell-derived neural progenitor cells

  5. STEMdiff™ Midbrain Neuron Differentiation Kit
    STEMdiff™ Midbrain Neuron Differentiation K...

    Differentiation kit for the generation of midbrain-patterned neuronal precursors from human ES and iPS cell-derived neural progenitor cells

Overview

Start your neural workflow confidently with high-quality, ready-to-use human neural progenitor cells (NPCs). These specially cryopreserved central nervous system (CNS)-type progenitors are differentiated from the human induced pluripotent stem cell (iPSC) control line, SCTi003-A, which is derived from healthy female donor peripheral blood mononuclear cells (PBMCs). Ready to use directly from thawing, these human NPCs are multipotent and suitable for customized downstream differentiation to various CNS cell types, such as forebrain neurons, midbrain neurons, and astrocytes, using STEMdiff™ kits.

To ensure high-quality cells, these NPCs were differentiated from the robust, extensively tested SCTi003-A line using serum-free STEMdiff™ SMADi Neural Induction Kit. NPCs can be expanded using STEMdiff™ Neural Progenitor Medium, allowing for scale-up and reducing the cost of workflows that require large numbers of cells. Cryopreserve expanded NPCs using STEMdiff™ Neural Progenitor Freezing Medium for flexibility in your experimental schedule.

This research-use-only (RUO) product has been consented for both academic and commercial use. Blood samples are ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For donor details and cell quality characterization of the source cell banks, refer to the data figures on this page. SCTi003-A is derived from an αβ T cell and has undergone VDJ sequence rearrangement. It is karyotypically stable, demonstrates trilineage differentiation potential, expresses undifferentiated cell markers, and was reprogrammed using a non-integrating reprogramming technology. Registration with hPSCreg® ensures ethical and biological conformity based on community standards. For additional details, refer to the lot-specific Certificate of Analysis and Frequently Asked Questions about iPSC lines.
Contains
STEMdiff™ Neural Progenitor Freezing Medium
Cell Type
Neural Stem and Progenitor Cells
Species
Human
Cell and Tissue Source
Pluripotent Stem Cells
Application
Cell Culture
Brand
STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing, Neuroscience
Donor Status
Normal

Data Figures

Thawed and plated Human iPSC-Derived Neural Progenitor Cells exhibit high-quality, characteristic NPC morphology.

Figure 1. Human iPSC-Derived Neural Progenitor Cells Exhibit High-Quality Morphology Characteristic of Multipotent Central Nervous System Progenitor Cells

Cryopreserved Human iPSC-Derived Neural Progenitor Cells were thawed and plated onto Corning® Matrigel®-coated plates at 200,000 cells/cm². NPCs were incubated for 24 hours in STEMdiff™ Neural Progenitor Medium at 37℃ and subsequently analyzed by brightfield microscopy. NPCs display the small, teardrop-shaped morphology expected for NPCs. (A) 4X magnification, (B) 10X magnification. iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells expressing neural progenitor markers SOX1 and PAX6, with low βIII-TUB expression.

Figure 2. Human iPSC-Derived Neural Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Neural Progenitor Cells generated from SCTi003-A iPSCs were thawed, established in culture, and fixed for immunocytochemistry. The NPCs express neural progenitor markers (A) SOX1 and (B) PAX6 with low expression of (C) βIII-TUB. (D) In addition, they display the expected small, teardrop-shaped morphology. (E) The percentage expression of these markers were quantified. Neural progenitor markers PAX6 and SOX1 were found to be expressed in 90% of NPCs, while mature neuronal marker βIII-TUB was expressed in less than 10% of NPCs. Error bars represent standard deviation (n = 2 biological replicates). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to forebrain neurons expressing neural identity marker βIII-TUB.

Figure 3. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Forebrain Neurons

Forebrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Forebrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Forebrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Forebrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting forebrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (magenta), (C) but not astrocyte marker GFAP (green). (D) Nuclei are labeled with DAPI (gray). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to midbrain neurons expressing neural identity marker, βIII-TUB, and dopaminergic neuron marker, TH, but not astrocyte marker, GFAP.

Figure 4. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Midbrain Neurons

Midbrain neurons were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Midbrain Neuron Differentiation Medium. NPCs were cultured for six days at 37℃ in STEMdiff™ Midbrain Neuron Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Midbrain Neuron Maturation medium for 14 days. Resulting cells were processed for immunocytochemistry. (A) The resulting midbrain neuron cultures contain a population of cells expressing (B) neuronal identity marker βIII-TUB (red) and (C) dopaminergic neuron marker TH (green), (D) but not astrocyte marker GFAP (magenta). (D) Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Immunocytochemistry images show Human iPSC-Derived Neural Progenitor Cells differentiated to astrocytes expressing astrocyte identity marker S100β and GFAP, but not neuronal marker DCX.

Figure 5. Human iPSC-Derived Neural Progenitor Cells Can Effectively Differentiate into Astrocytes

Astrocytes were generated from Human iPSC-Derived Neural Progenitor Cells using STEMdiff™ Astrocyte Differentiation Medium. NPCs were cultured for 21 days at 37℃ in STEMdiff™ Astrocyte Differentiation Medium. Cells were subsequently cultured in STEMdiff™ Astrocyte Maturation medium for seven days. Resulting cells were processed for immunocytochemistry. (A) The resulting astrocyte cultures contain a population of cells expressing (B) astrocyte marker S100β (green) and (C) GFAP (red), but not neuronal marker DCX (magenta). Nuclei are labeled with DAPI (blue). iPSC = induced pluripotent stem cell; NPCs = neural progenitor cells.

Bar graph showing expansion rate of Human iPSC-Derived Neural Progenitor Cells to 5 passages.

Figure 6. Human iPSC-Derived Neural Progenitor Cells Can Be Expanded for Multiple Passages

Human iPSC-Derived Neural Progenitor Cells were thawed and maintained in STEMdiff™ Neural Progenitor Medium at 37℃ and passaged roughly once every seven days for a total of five weeks. The average fold increase of NPCs was 2.8 ± 0.5 (mean ± SEM) per passage, demonstrating cells can be effectively expanded.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Neural Progenitor Cells
Catalog #
200-0620, 200-0621
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Resources and Publications

Educational Materials (9)

hPSC-Derived Neural Cell Research
Brochure
hPSC-Derived Neural Cell Research
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
STEMCELL Neural Product Portfolio
Brochure
STEMCELL Neural Product Portfolio
Directed Differentiation of Pluripotent Stem Cells
Wallchart
Directed Differentiation of Pluripotent Stem Cells
Cell-Reprogramming Technology and Neuroscience
Wallchart
Cell-Reprogramming Technology and Neuroscience
Modeling Human Neurological Disease with Induced Pluripotent Stem Cells
1:05:46
Webinar
Modeling Human Neurological Disease with Induced Pluripotent Stem Cells
Highly Characterized Human iPSCs and NPCs for Downstream Differentiation Applications
30:53
Webinar
Highly Characterized Human iPSCs and NPCs for Downstream Differentiation Applications
Development of iPSCs, Differentiated Cells, and Organoids Under Stringent Quality Standards
13:32
Webinar
Development of iPSCs, Differentiated Cells, and Organoids Under Stringent Quality Standards
The Downstream Differentiation Potential of Human Induced Pluripotent Stem Cell-Derived Neural Progenitor Cells to Forebrain Neurons and Astrocytes
Scientific Poster
The Downstream Differentiation Potential of Human Induced Pluripotent Stem Cell-Derived Neural Proge...

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Legal Statement:

These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. Copyright © 2023 by STEMCELL Technologies Inc. All rights reserved including graphics and images. STEMCELL Technologies & Design, STEMCELL Shield Design, Scientists Helping Scientists, CloneR, ReLeSR, and STEMdiff are trademarks of STEMCELL Technologies Canada Inc. mTeSR and TeSR are trademarks of WARF. Corning, Falcon, and Matrigel are registered trademarks of Corning Incorporated. CryoStor and ThawStar are registered trademarks of BioLife Solutions. All other trademarks are the property of their respective holders. While STEMCELL has made all reasonable efforts to ensure that the information provided by STEMCELL and its suppliers is correct, it makes no warranties or representations as to the accuracy or completeness of such information.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.

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