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Human iPSC-Derived Mesenchymal Progenitor Cells

Frozen human mesenchymal progenitor cells differentiated from human induced pluripotent stem cell line, SCTi003-A

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Human iPSC-Derived Mesenchymal Progenitor Cells

Frozen human mesenchymal progenitor cells differentiated from human induced pluripotent stem cell line, SCTi003-A

From: 799 USD
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Frozen human mesenchymal progenitor cells differentiated from human induced pluripotent stem cell line, SCTi003-A
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Product Advantages


  • Save time by starting your experiments with a highly characterized population of mesenchymal progenitor intermediates

  • Obtain high-quality MPCs, derived from the highly characterized hiPSC control line, SCTi003-A

  • Start your cultures immediately post-thaw, no cell recovery culture required

  • Differentiation into adipocytes, osteoblasts, or chondrocytes with compatible MesenCult™ differentiation systems

What's Included

Human iPSC-Derived Mesenchymal Progenitor Cells (Catalog #200-0781)
Products for Your Protocol
To see all required products for your protocol, please consult the Protocols and Documentation.
  1. Animal Component-Free Cell Dissociation Kit
    Animal Component-Free Cell Dissociation Kit

    Dissociation kit for human stem and progenitor cells

  2. MesenCult™-ACF Plus Culture Kit
    MesenCult™-ACF Plus Culture Kit

    Animal component-free medium and attachment substrate for human mesenchymal stem cells

  3. MesenCult™-ACF Freezing Medium
    MesenCult™-ACF Freezing Medium

    Animal component-free MSC cryopreservation medium

  4. L-Glutamine
    L-Glutamine

    Cell culture supplement

Overview

Start your mesenchymal cultures confidently with high-quality, ready-to-use Human iPSC-Derived Mesenchymal Progenitor Cells (MPCs). These cryopreserved multipotent progenitor cells are differentiated from the human induced pluripotent stem cell (iPSC) control line, SCTi003-A, derived from healthy female donor peripheral blood mononuclear cells (PBMCs). Ready to use directly from thawing, MPCs are multipotent and can be reliably cultured and differentiated using our MesenCult™ media line. The MPCs readily differentiate into cells of the mesenchymal lineage, such as adipocytes (MesenCult™ Adipogenic Differentiation Kit [Human]), osteoblasts (MesenCult™ Osteogenic Differentiation Kit [Human]), and chondrocytes (MesenCult™ Chondrogenic Differentiation Kit [Human]).

To ensure high quality, Human iPSC-Derived MPCs were differentiated from SCTi003-A cells using STEMdiff™ Mesenchymal Progenitor Kit. The resultant MPCs express high levels of mesenchymal cell markers, such as CD73, CD90, and CD105, and are capable of long-term expansion when cultured with MesenCult™-ACF Plus Medium (also included in MesenCult™-ACF Plus Culture Kit). The MPCs may also be cryopreserved for storage using MesenCult™-ACF Freezing Medium, allowing for flexibility in your workflow. MPCs, as well as the adipogenic, osteogenic, and chondrogenic cells generated from them, can be used for studying regenerative medicine development, drug discovery, and disease modeling.

This research-use-only (RUO) product has been approved for both academic and commercial use. Blood samples are ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For donor details and cell quality characterization of the source cell banks, refer to the data figures on this page. For additional details, refer to Frequently Asked Questions about iPSC lines.
Contains
CryoStor® CS10
Subtype
Frozen
Cell Type
Mesenchymal Stem and Progenitor Cells
Species
Human
Cell and Tissue Source
Pluripotent Stem Cells
Application
Cell Culture, Expansion
Area of Interest
Disease Modeling, Extracellular Vesicle Research, Stem Cell Biology
Donor Status
Normal
Purity
≥ 90% CD73+CD90+CD105+ cells by flow cytometry

Data Figures

Figure 1

Figure 1. Human iPSC-Derived Mesenchymal Progenitor Cells Show High-Quality Morphology and Robust Expansion for Multiple Passages

(A) Representative microscopy image showing cryopreserved Human iPSC-Derived Mesenchymal Progenitor Cells thawed and maintained in MesenCult™-ACF Plus Medium for 4 days and ready for passaging at ~80% confluency. (B) Cells show robust expansion post-thaw. Data points show the average doubling time over 3 passages post-thaw from 3 technical replicates. C) Human iPSC-Derived Mesenchymal Progenitor Cells were thawed and maintained in MesenCult™-ACF Plus Medium. Cells were passaged every 3-4 days post thaw and cell expansion was analyzed as cumulative cell number over 4 passages from 2 technical replicates.

Figure 2

Figure 2. Human iPSC-Derived Mesenchymal Progenitor Cells Express Characteristic Markers

Human iPSC-Derived Mesenchymal Progenitor Cells were thawed, cultured in MesenCult™-ACF Plus Medium for one passage, characterized using flow cytometry for mesenchymal cell markers, and show high expression of CD73, CD90, and CD105. (A) Percentage marker expression was quantified from the analyses of five technical replicates. Representative flow cytometry plots are displayed for (B) CD73, (C) CD90 and (D) CD105.

Figure 3

Figure 3. Human iPSC-Derived Mesenchymal Progenitor Cells Can Differentiate into Adipocytes, Osteoblasts, and Chondrocytes

(A) Adipocytes were generated from Human iPSC-Derived Mesenchymal Progenitor Cells using MesenCult™ Adipogenic Differentiation Kit. Adipogenic differentiation was assessed by Oil Red O stain at Day 30 post differentiation. (B) Osteoblasts were differentiated using MesenCult™ Osteogenic Differentiation Kit. Osteogenic differentiation was assessed by Alizarin Red S stain at Day 21 post differentiation. (C) Chondrocytes were differentiated using MesenCult™-ACF Chondrogenic Differentiation Kit. Chondrogenic differentiation was assessed by Alcian Blue and Nuclear Fast Red Stain at Day 21 post differentiation.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human iPSC-Derived Mesenchymal Progenitor Cells
Catalog #
200-0781
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

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Legal Statement:

These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) shall not be used or administered in (1) human subjects for human clinical use; (2) animals for veterinary use for therapeutic, diagnostic, or prophylactic purposes or (3) any subject in relation to clinical applications, cell therapy, transplantation, and/or regenerative medicines, without limiting the generality of the foregoing. These iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for monetization or commercialization purposes, including without limitation, used to, or with the goal to, perform services or supply products or rights, including in the manufacture of cellular therapies or other therapeutics, for monetary gain or the generation of royalties, revenues, sales or other valuable consideration. For clarity, these iPSCs and their modifications (including but not limited to derivatives or differentiated progeny) may not be used for screening compounds, antibodies, proteins or peptides, except for the purposes of target discovery, target validation, or assay development, provided such activities and the results of such activities are not further used for monetization or commercialization purposes. It may be possible to obtain a further license for the prohibited uses referred to in this Limited Use License. Please contact iPSCrequests@stemcell.com for more details.

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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