Human iPSC-Derived Hepatic Organoids

Frozen human hepatocyte-like cell-derived organoids generated from human induced pluripotent stem cell lines

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Human iPSC-Derived Hepatic Organoids

Frozen human hepatocyte-like cell-derived organoids generated from human induced pluripotent stem cell lines

From: 1,499 USD

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Frozen human hepatocyte-like cell-derived organoids generated from human induced pluripotent stem cell lines

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Product Advantages

Save time with pre-established, cryopreserved hepatic organoids derived from well-characterized hiPSC control lines
Streamline your workflow with liver organoids ready for immediate post-thaw expansion prior to terminal differentiation
Scale your cultures efficiently by expanding organoids post-thaw using STEMdiff™ Hepatic Organoid Growth Medium
Obtain physiologically relevant results with hepatic organoids expressing key mature liver-specific markers and exhibiting critical hepatic functions upon differentiation
Model liver biology, assess drug-induced liver injury, and evaluate human-specific metabolic profiles in a physiologically relevant 3D system

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To see all required products for your protocol, please consult the Protocols and Documentation.

STEMdiff™ Hepatic Organoid Media
Serum-free cell culture media for the growth and differentiation of hepatic organoids from hPSC-derived hepatocyte-like cells
Organoid Culture Plates
Cell culture plates for easy and reproducible generation of organoids
Labeling Antibodies
Compatible antibodies for purity assessment of isolated cells

Overview

Advance your liver research with confidence using high-quality, cryopreserved Human Induced Pluripotent Stem Cell (iPSC)-Derived Hepatic Organoids. Derived from well-characterized healthy control iPSC lines, these three-dimensional in vitro organotypic models provide a human-relevant system for studying hepatic development, drug-induced liver injury (DILI), hepatotoxicity, and metabolism. Human iPSC-Derived Hepatic Organoids are generated using STEMdiff™ Hepatocyte Kit (Catalog #100-0520) and established in STEMdiff™ Hepatic Organoid Growth Medium (Catalog #100-1773). Post-thaw, they can be recovered and expanded for several passages to support scalable applications. For downstream maturation, use STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) to yield hepatic organoids that express mature hepatocyte markers (e.g. ALB, CYP3A4, ASGR1, and CPS1) and exhibit key functional characteristics, including albumin secretion and CYP3A4 activity.

Consent has been given for both academic and commercial use of this research-use-only (RUO) product. Original donor blood samples were ethically sourced using Institutional Review Board (IRB) or other regulatory authority-approved consent forms and protocols. For additional details, refer to the lot-specific Certificate of Analysis and Frequently Asked Questions about iPSC lines.

Certain products are only available in select territories. Please contact your sales representative or Product & Scientific Support for further information.

Frozen

Hepatic Cells, PSC-Derived, Pluripotent Stem Cells

Human

Pluripotent Stem Cells

Cell Culture, Differentiation, Functional Assay, Genome Editing, Toxicity Assay

Cell Line Development, Disease Modeling, Drug Discovery and Toxicity Testing, Organoids, Stem Cell Biology

Data Figures

Figure 1. Workflow for Recovery, Expansion, and Differentiation of Human iPSC-Derived Hepatic Organoids

(A) Human iPSC-derived hepatic organoid fragments can be thawed, seeded in Matrigel® domes, and cultured in STEMdiff™ Hepatic Organoid Growth Medium (Catalog #100-1773) to generate confluent cultures within 7 days. Organoids can then be expanded every 6 - 10 days via fragment passaging in STEMdiff™ Hepatic Organoid Growth Medium. (B) After at least one passage post-thaw, cultures can be switched to STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) to differentiate organoids toward more mature hepatic cell types. iPSC = induced pluripotent stem cell

Figure 2. Human iPSC-Derived Hepatic Organoids Exhibit Efficient Post-Thaw Recovery and Expansion

Human iPSC-Derived Hepatic Organoids demonstrate robust (A) post-thaw recovery of organoid fragments (N = 11 experiments) and (B) efficient expansion across 9 passages. Human iPSC-Derived Hepatic Organoids derived from SCTi003-A were thawed and expanded in STEMdiff™ Organoid Growth Medium (Catalog #100-1773), and cumulative fragment yields were quantified over 9 passages (N = 5 experiments). Error bars = SD. iPSC = induced pluripotent stem cell

Figure 3. Human iPSC-Derived Hepatic Organoids Can Be Differentiated for Use in Downstream Assays

Representative images of Human iPSC-Derived Hepatic Organoids (A) thawed and cultured in STEMdiff™ Hepatic Organoid Growth Medium (OGM; Catalog #100-1773) for 7 days, and (B) following 10 days of differentiation using STEMdiff™ Hepatic Organoid Differentiation Medium (ODM; Catalog #100-1774). iPSC = induced pluripotent stem cell

Figure 4. Differentiated Human iPSC-Derived Hepatic Organoids Express Key Mature Hepatocyte Markers

Representative immunofluorescence images of Human iPSC-Derived Hepatic Organoids derived from SCTi003-A that were differentiated using STEMdiff™ Hepatic Organoid Differentiation Medium (Catalog #100-1774) for 10 days. Differentiated hepatic organoids express hepatic transcription factor (A, B) HNF4A, and mature hepatocyte markers (A) ALB, (B, C, D) A1AT, and (C) BSEP. Ductal marker (D) KRT19 and epithelial marker (B) ECAD are also expressed, indicating a hepatobiliary phenotype. Nuclei are counterstained with DAPI. iPSC = induced pluripotent stem cell; ALB = albumin; A1AT = alpha-1 antitrypsin; BSEP = bile salt export pump; HNF4A = hepatocyte nuclear factor 4 alpha; KRT19 = cytokeratin 19; ECAD = e-cadherin