EasySep™ EasyStand™

Linkable stand for EasySep™ Magnet
EasySep™ EasyStand™

One linkable stand for EasySep™ Magnet

1 Each
Catalog # 18130
401 USD
EasySep™ EasyStand™ with Magnet

One linkable stand and one EasySep™ Magnet

1 Each
Catalog # 18131
1,179 USD
EasySep™ EasyStand™ x 4

Four linkable stands for EasySep™ Magnets

1 Each
Catalog # 18134
1,604 USD
EasySep™ EasyStand™ x 4 with Magnets

Four linkable stands and four EasySep™ Magnets

1 Each
Catalog # 18135
4,717 USD
EasySep™ EasyStand™ x 6

Six linkable stands for EasySep™ Magnets

1 Each
Catalog # 18136
2,407.02 USD
EasySep™ EasyStand™ x 6 with Magnets

Six linkable stands and six EasySep™ Magnets

1 Each
Catalog # 18137
7,075.02 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

Overview

The EasySep™ EasyStand™ facilitates the separation of one or more samples at one time. Each EasySep™ EasyStand™ can hold a single EasySep™ Magnet (Catalog #18000), and a simple system links up to 6 individual EasySep™ EasyStands™ together. After each round of magnetic separation, the supernatants from all samples are poured off simultaneously into the receiving tubes.
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Cell Isolation
Brand
EasySep

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ EasyStand™
Catalog #
18130
Lot #
All
Language
English

Educational Materials(3)

Brochure
Tools For Your Immunology Research
Video
1:37
How to Isolate PBMCs from Whole Blood Using Density Gradient Centrifugation (Ficoll™ or Lymphoprep™)
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology

Frequently Asked Question

Can EasySep™ be used for either positive or negative selection?

Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).

How does the separation work?

Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.

Which columns do I use?

The EasySep™ procedure is column-free. That's right - no columns!

How can I analyze the purity of my enriched sample?

The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.

Can EasySep™ separations be automated?

Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.

Can EasySep™ be used to isolate rare cells?

Yes. We recommend a cell concentration of 2x108 cells/mL and a minimum working volume of 100 µL. Samples containing 2x107 cells or fewer should be suspended in 100 µL of buffer.

Are the EasySep™ magnetic particles FACS-compatible?

Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.

Can the EasySep™ magnetic particles be removed after enrichment?

No, but due to the small size of these particles, they will not interfere with downstream applications.

Can I alter the separation time in the magnet?

Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.

For positive selection, can I perform more than 3 separations to increase purity?

Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

How does the binding of the EasySep™ magnetic particle affect the cells? is the function of positively selected cells altered by the bound particles?

Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.

If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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