RoboSep™-C
Automated closed-system immunomagnetic cell isolation instrument
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RoboSep™-C Tubing SetFor automated cell isolation with RoboSep™-C
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RoboSep™-C PBS/EDTA BufferCell separation buffer
Overview
Incorporating this dedicated cell separation instrument into your workflows can free-up your equipment and resources, enabling you to streamline and scale up your sample processing operations while maintaining flexibility and compatibility for downstream assays. RoboSep™-C reduces hands-on time and operator error by automating cell isolations from large-volume apheresis products and uses the same column-free immunomagnetic technology as EasySep™. The closed system performs all necessary cell processing steps—including sample washing, buffer exchange, and concentration—so you can efficiently isolate highly purified and functional cells in a sterile environment.
Why Use RoboSep™-C?
• EFFICIENT. Automate all cell processing, cell washing, sample labeling, and immunomagnetic cell isolation steps in as little as 75 minutes.
• ADAPTABLE. Easily integrate cell isolation into your established workflow while maintaining flexibility for downstream assays.
• STERILE. Isolate cells in a closed, sterile fluid path using a single-use tubing set.
• SCALABLE. Increase your lab throughput by performing cell isolation from multiple full-sized leukopaks in a single day.
For more information about Instrument Services including additional service packages, please see our instrumentation overview.
Data Figures
Figure 1. High Purity of T Cells Following Isolation with RoboSep™-C
T cells were isolated from Fresh Human Peripheral Blood Leukopaks (between 2.5 - 20 x 109 starting cells) by negative selection. RoboSep™-C isolation yielded high purity of T cells, CD4+ T cells, and CD8+ T cells. Data shown as mean ± SD; n = 12 - 16.
Figure 2. Cell Isolation with RoboSep™-C Yields High Recovery of T Cells from a Leukopak
T cells were isolated from Fresh Human Peripheral Blood Leukopaks by negative selection. RoboSep™-C isolation yielded a high recovery of a) T cells, b) CD4+ T cells, and c) CD8+ T cells. Data shows the number of target cells recovered per 10 x 109 starting cells (mean ± SD; n= 12 - 16).
Figure 3. Cells Isolated Using RoboSep™-C Show Comparable Viability to Starting Samples
T cells were isolated from Fresh Human Peripheral Blood Leukopaks using RoboSep™-C isolation kits. Pre- and post-isolation samples were stained with the cell viability dye DRAQ7™ and appropriate cell surface markers and were assessed by flow cytometry. Cells isolated using RoboSep™-C showed no significant difference in viability (% DRAQ7-negative events) compared to the starting samples. Data shown as mean ± SD; n = 12 - 16.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (9)
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Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
