DNA Ladder & Loading Dye

For size determination and tracking of double-stranded DNA products by gel electrophoresis
DNA Loading Dye

Ready-to-use marker dye for loading DNA samples into agarose gel wells and tracking migration during electrophoresis

3 x 1 mL
Catalog # 79018
60 USD
1 kb DNA Ladder

DNA ladder consisting of 13 fragments from 250 - 10,000 base pairs for determining the size of double-stranded DNA by gel electrophoresis

600 µL
Catalog # 79017
129 USD

Overview

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 - 10000 base pairs (bp) by gel electrophoresis. The ladder consists of 13 double-stranded, blunt-end DNA fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1). The 1000 and 3000 bp fragments have greater intensity relative to the other bands for easy identification. DNA Ladder is not intended for quantitative analysis. The 1 kb DNA ladder comes pre-mixed with DNA Loading Dye, which is also available for individual sale (Catalog #79018), for loading DNA samples into agarose gel wells. The recommended loading volume of the 1 kb DNA ladder is 6 μL per lane.

DNA Loading Dye is a ready-to-use marker dye containing orange G (0.4%), bromophenol blue (0.03%), and xylene cyanol FF (0.03%), in 15% Ficoll® 400, 10 mM Tris-HCl (pH 7.5), and 50 mM EDTA (pH 8.0). The dye is used for loading DNA samples into agarose gel wells and tracking migration during electrophoresis. In a 0.5 - 1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp, and orange G at approximately 50 bp. This product is free of nuclease activity.
Subtype
Genome Editing
Species
Human, Mouse, Rat
Area of Interest
Disease Modeling

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
DNA Loading Dye
Catalog #
79018
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
1 kb DNA Ladder
Catalog #
79017
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
DNA Loading Dye
Catalog #
79018
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
1 kb DNA Ladder
Catalog #
79017
Lot #
All
Language
English

Educational Materials (2)

Technical Bulletin
Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System
Technical Bulletin
Genome Editing with Direct Cas9 RNP Delivery Design Considerations

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.