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DNA Ladder & Loading Dye

For size determination and tracking of double-stranded DNA products by gel electrophoresis

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1 kb DNA Ladder

DNA ladder consisting of 13 fragments from 250 - 10,000 base pairs for determining the size of double-stranded DNA by gel electrophoresis

600 µL
Catalog #79017
119 USD

DNA Loading Dye

Ready-to-use marker dye for loading DNA samples into agarose gel wells and tracking migration during electrophoresis

3 x 1 mL
Catalog #79018
55 USD

Overview

1 kb DNA Ladder allows for determining the size of double-stranded DNA from 250 - 10000 base pairs (bp) by gel electrophoresis. The ladder consists of 13 double-stranded, blunt-end DNA fragments, of sizes 250, 500, 750, 1000, 1500, 2000, 2500, 3000, 4000, 5000, 6000, 8000, and 10,000 bp (see Figure 1). The 1000 and 3000 bp fragments have greater intensity relative to the other bands for easy identification. DNA Ladder is not intended for quantitative analysis. The 1 kb DNA ladder comes pre-mixed with DNA Loading Dye, which is also available for individual sale (Catalog #79018), for loading DNA samples into agarose gel wells. The recommended loading volume of the 1 kb DNA ladder is 6 μL per lane.

DNA Loading Dye is a ready-to-use marker dye containing orange G (0.4%), bromophenol blue (0.03%), and xylene cyanol FF (0.03%), in 15% Ficoll® 400, 10 mM Tris-HCl (pH 7.5), and 50 mM EDTA (pH 8.0). The dye is used for loading DNA samples into agarose gel wells and tracking migration during electrophoresis. In a 0.5 - 1.4% agarose gel in 0.5X TBE, xylene cyanol FF migrates at approximately 4 kb, bromophenol blue at approximately 300 bp, and orange G at approximately 50 bp. This product is free of nuclease activity.
Subtype:
Genome Editing
Species:
Human; Mouse; Rat
Area of Interest:
Disease Modeling

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STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.