ArciTect™ Cas9 Nuclease

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Overview

Related Products

Scientific Resources

Product Applications

Overview

ArciTect™ Cas9 Nuclease requires association with a guide RNA—e.g. ArciTect™ sgRNA (Catalog #200-0013) or a duplex composed of ArciTect™ tracrRNA (Catalog #76016) and ArciTect™ crRNA (Catalog #76010)—to form a ribonucleoprotein (RNP) complex. This RNP complex then creates double-strand breaks at site-specific locations in the genome. ArciTect™ Cas9 Nuclease contains a nuclear localization signal at the N-terminus, ensuring that the RNP complex translocates to the nucleus, thereby increasing the efficiency of genome editing. As the RNP complex is fully functional upon transfection, it allows for immediate activity following translocation to the nucleus. The RNP complex is degraded over 48 hours, allowing sufficient time for genome editing to occur while reducing off-target effects that can be caused by the continuous presence of the RNP complex. Using the RNP system also circumvents the laborious process of generating stable Cas9-expressing cell lines, saving time and reducing the risk of off-target effects due to leaky inducible expression systems. The S. pyogenes Cas9 uses the protospacer adjacent motif (PAM) sequence NGG (where N can be any nucleotide). The enzyme will not cleave without a genomic PAM site downstream of the target sequence.

Cell Type

Pluripotent Stem Cells

Species

Human

Brand

ArciTect

Area of Interest

Stem Cell Biology

Related Products

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1. 

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   For estimation of CRISPR-Cas9 genome editing efficiency

2. 

   CloneR™

   Defined supplement for single-cell cloning of human ES and iPS cells

Scientific Resources

Product Documentation

Document Type	Product Name	Catalog #	Lot #	Language
Document Type Product Information Sheet	Product Name ArciTect™ Cas9 Nuclease	Catalog # 76002, 76004	Lot # All	Language English
Document Type Safety Data Sheet	Product Name ArciTect™ Cas9 Nuclease	Catalog # 76002, 76004	Lot # All	Language English

Educational Materials (12)

Brochure

ArciTect™ CRISPR-Cas9 Genome Editing System

Technical Bulletin

Evaluation of Genome Editing

Technical Bulletin

Genome Editing of Human Primary T Cells Using CRISPR-Cas9

Technical Bulletin

Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System

Technical Bulletin

Genome Editing with Direct Cas9 RNP Delivery Design Considerations

Protocol

CRISPR-Cas9 Genome Editing of Human Intestinal Organoids

Webinar

10:08

CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly

Webinar

57:20

Improving Genome Editing with Enhanced CRISPR-Cas Nucleases

Webinar

55:51

CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells

Webinar

1:07:14

Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types

Webinar

50:49

hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling

Scientific Poster

Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modification and Overall Cell Yield

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area

Workflow Stages

Workflow Stages for Human Pluripotent Stem Cell Research

Reprogramming

Maintenance

Differentiation

Workflow Stages for Genome Editing

View All

Workflow Stages for T Cell Engineering

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

Cell Cryopreservation

Workflow Stages for T Cell Research

Cell Sourcing

Cell Isolation

Cell Activation, Expansion, and Differentiation

Cell Characterization

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ArciTect™ Cas9 Nuclease

Legal Statement:

The purchase of the ArciTect™ products conveys to the purchaser the limited, non-transferable right to use, in accordance with all applicable laws and regulations, the ArciTect™ products and any related material solely for research purposes only, not for any clinical, human, agricultural, veterinary, livestock, or commercial purpose, with no warranty (express or implied) from the owners or assignees of the Patents or STEMCELL Technologies each of whom expressly disclaim any warranty regarding results obtained through the use of the ArciTect™ products, and without any exception the owners or assignees of the Patents or STEMCELL Technologies, or as applicable a director, trustee, officer, employee, agent, faculty, official investigator or student thereof, will not suffer or be exposed to any liability, damages, loss, or expense of any kind whatsoever arising from or related to the use of the ArciTect™ products by a purchaser of same. Distribution of ArciTect™ products by STEMCELL Technologies is covered under at least US 8,697,359, US 8,771,945, US 8,795,965, US 8,865,406, US 8,871,445, US 8,889,356, US 8,889,418, US 8,895,308, US 8,906,616 and foreign equivalents (“Patents”).

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.