ArciTect™ Cas9 Nuclease

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

ArciTect™ Cas9 Nuclease

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

From: 291 USD
Catalog #
76001_C
Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
Products for Your Protocol
To see all required products for your protocol, please consult the Product Information Sheet.

Overview

ArciTect™ Cas9 Nuclease requires association with a guide RNA—e.g. ArciTect™ sgRNA (Catalog #200-0013) or a duplex composed of ArciTect™ tracrRNA (Catalog #76016) and ArciTect™ crRNA (Catalog #76010)—to form a ribonucleoprotein (RNP) complex. This RNP complex then creates double-strand breaks at site-specific locations in the genome. ArciTect™ Cas9 Nuclease contains a nuclear localization signal at the N-terminus, ensuring that the RNP complex translocates to the nucleus, thereby increasing the efficiency of genome editing. As the RNP complex is fully functional upon transfection, it allows for immediate activity following translocation to the nucleus. The RNP complex is degraded over 48 hours, allowing sufficient time for genome editing to occur while reducing off-target effects that can be caused by the continuous presence of the RNP complex. Using the RNP system also circumvents the laborious process of generating stable Cas9-expressing cell lines, saving time and reducing the risk of off-target effects due to leaky inducible expression systems. The S. pyogenes Cas9 uses the protospacer adjacent motif (PAM) sequence NGG (where N can be any nucleotide). The enzyme will not cleave without a genomic PAM site downstream of the target sequence.
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
76002, 76004
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
76002, 76004
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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