ArciTect™ Cas9 Nuclease

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
Catalog #
76001_C
Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing
From: 215 EUR
Required Products
  1. ArciTect™ crRNA
    ArciTect™ crRNA

    Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing

  2. ArciTect™ tracrRNA Kit
    ArciTect™ tracrRNA Kit

    Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

  3. Nuclease-Free Water|79001
    Nuclease-Free Water

    DNase- and RNase-free water for molecular biology applications

  4. ArciTect™ sgRNA
    ArciTect™ sgRNA

    Custom-designed single guide RNA for CRISPR-Cas9 genome editing

Overview

ArciTect™ Cas9 Nuclease requires association with a guide RNA—e.g. ArciTect™ sgRNA (Catalog #200-0013) or a duplex composed of ArciTect™ tracrRNA (Catalog #76016) and ArciTect™ crRNA (Catalog #76010)—to form a ribonucleoprotein (RNP) complex. This RNP complex then creates double-strand breaks at site-specific locations in the genome. ArciTect™ Cas9 Nuclease contains a nuclear localization signal at the N-terminus, ensuring that the RNP complex translocates to the nucleus, thereby increasing the efficiency of genome editing. As the RNP complex is fully functional upon transfection, it allows for immediate activity following translocation to the nucleus. The RNP complex is degraded over 48 hours, allowing sufficient time for genome editing to occur while reducing off-target effects that can be caused by the continuous presence of the RNP complex. Using the RNP system also circumvents the laborious process of generating stable Cas9-expressing cell lines, saving time and reducing the risk of off-target effects due to leaky inducible expression systems. The S. pyogenes Cas9 uses the protospacer adjacent motif (PAM) sequence NGG (where N can be any nucleotide). The enzyme will not cleave without a genomic PAM site downstream of the target sequence.
Cell Type
Pluripotent Stem Cells
Species
Human
Brand
ArciTect
Area of Interest
Stem Cell Biology

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
ArciTect™ Cas9 Nuclease
Catalog #
76002, 76004
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
ArciTect™ Cas9 Nuclease
Catalog #
76002, 76004
Lot #
All
Language
English

Educational Materials (12)

Brochure
ArciTect™ CRISPR-Cas9 Genome Editing System
Technical Bulletin
Genome Editing with Direct Cas9 RNP Delivery Design Considerations
Technical Bulletin
Evaluation of Genome Editing
Technical Bulletin
Genome Editing of Human Primary T Cells Using CRISPR-Cas9
Technical Bulletin
Genome Editing of Human Pluripotent Stem Cells Using the ArciTect™ CRISPR-Cas9 System
Protocol
CRISPR-Cas9 Genome Editing of Human Intestinal Organoids
Webinar
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
1:07:14
Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Webinar
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
10:08
CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
Webinar
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
57:20
Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
Webinar
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells
55:51
CRISPR-Cas9 Editing of Hematopoietic Stem and Progenitor Cells
Scientific Poster
Optimization of Human T Cell Activation and Expansion Protocols Improves Efficiency of Genetic Modification and Overall Cell Yield

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

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