Genome Editing and Molecular Tools

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

Modified Cas9 nuclease for the generation of single-strand breaks in CRISPR-Cas9 genome editing

Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing

Positive control for CRISPR-Cas9 genome editing

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

For guide RNA generation in CRISPR-Cas9 genome editing

For estimation of CRISPR-Cas9 genome editing efficiency

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification

For size determination and tracking of double-stranded DNA products by gel electrophoresis

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages