Genome Editing and Molecular Tools

Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

Enhanced green fluorescent protein (eGFP)-tagged Cas9 nuclease for the generation of double-strand breaks in CRISPR-Cas9 genome editing

Modified Cas9 nuclease for the generation of single-strand breaks in CRISPR-Cas9 genome editing

Custom-designed CRISPR RNA for guide RNA generation in CRISPR-Cas9 genome editing

Positive control for CRISPR-Cas9 genome editing

Trans-activating crRNA for guide RNA generation in CRISPR-Cas9 genome editing

For guide RNA generation in CRISPR-Cas9 genome editing

For estimation of CRISPR-Cas9 genome editing efficiency

For purification of genomic DNA from cells or tissue

For extraction of DNA from agarose gels, or clean-up of PCR products and restriction enzyme digestions

For purification of RNA from cells

Polymerase, buffers, and dNTPs for high-fidelity PCR amplification

For size determination and tracking of double-stranded DNA products by gel electrophoresis

For characterization of human MSCs and their differentiation to chondrogenic, adipogenic, or osteogenic lineages

For characterizaton of hPSC-derived progenitor cells of definitive endoderm, hepatic, intestinal, or pancreatic lineages

For characterization of gene expression associated with hPSC naïve or primed states

For characterization of hPSC trilineage differentiation to endoderm, mesoderm, and ectoderm germ layers

DNase- and RNase-free water for molecular biology applications

For removing nucleases from DNA/RNA samples and terminating enzymatic reactions

For reverse transcription of RNA before qPCR or RNA sequencing

DNA polymerase master mix and nuclease-free water for PCR reactions

Hot Start DNA polymerase master mix and nuclease-free water for PCR reactions at room temperature

qPCR analysis kit for detecting the majority of karyotypic abnormalities reported in human ES and iPS cells