Mouse IL-12 (p70) ELISA Kit

For detection and measurement of mouse interleukin 12 p70 subunit

More Views

From: 575 USD

Options

* Required Fields

Catalog # (Select a product)
For detection and measurement of mouse interleukin 12 p70 subunit
From: 575 USD

Overview

The Mouse Interleukin 12 (IL-12) p70 ELISA Kit is designed for the quantitative detection and measurement of mouse IL-12 (p70) in biological fluids such as serum, plasma, and cell culture supernatants. IL-12 (p70) is a heterodimeric cytokine composed of p35 and p40 subunits; the p40 subunit is shared with IL-23. IL-12 is produced by monocytes, macrophages, dendritic cells, neutrophils, and B cells. IL-12 is a regulator of cytokine synthesis, especially IFN-γ, enhances the proliferation of T and NK cells, and stimulates the differentiation of CD4+ and CD8+ T cells.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components:
  • Mouse IL-12 (p70) ELISA Kit, 2 plates (Catalog #02026)
    • Mouse IL-12 (p70) ELISA Plate, 2x
    • Mouse IL-12 & IL-12/-23 Standard, 1 vial
    • Mouse IL-12 (p70) Biotinylated Detection Antibody, 30 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Mouse IL-12 (p70) ELISA Kit, 10 plates (Catalog #02027)
    • Mouse IL-12 (p70) ELISA Plate, 10x
    • Mouse IL-12 & IL-12/-23 Standard, 1 vial
    • Mouse IL-12 (p70) Biotinylated Detection Antibody, 130 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer B, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype:
Complete Kits
Cell Type:
B Cells; Dendritic Cells; Granulocytes and Subsets; Macrophages; Monocytes; T Cells
Species:
Mouse
Application:
ELISA
Area of Interest:
Immunology

Scientific Resources

Educational Materials

(2)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable range: 10 - 3160 pg/mL. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity.
• Sensitivity: The limit of detection of this assay is 6 pg/mL. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Accuracy: No international standard exists for calibration.
• Recovery: A mid-curve recovery of 85 - 121% was determined by spiking defined amounts of analyte standard into serum or plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.7% (CV). The inter-assay precision of this assay is 6.8% (CV).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.