Human Latent TGF-beta 1 ELISA Kit

For detection and measurement of human latent transforming growth factor-beta 1

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From: 800 USD

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For detection and measurement of human latent transforming growth factor-beta 1
From: 800 USD

Overview

The Human Latent Transforming Growth Factor-beta 1 (TGF-β1) Kit is designed for the quantitative detection and measurement of latent human TGF-β1 in biological fluids such as plasma and cell culture supernatants. TGF-β1 is produced primarily by platelets and bone, and in smaller amounts by most nucleated cells and tumors. TGF-β1 stimulates cells of mesenchymal origin, but inhibits cells of epithelial or neuroectodermal origin. TGF-β1 associates with Latency Associated Protein (LAP) to form a latent complex. This kit detects the LAP entity of the complex.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for the cytokine. The captured cytokine is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of cytokine in the sample. The concentration of the cytokine is determined by comparison to a serial dilution of the cytokine standard analyzed in parallel.
Components:
  • Human Latent TGF-beta 1 ELISA Kit, 2 plates (Catalog #02018)
    • Human Latent TGF-β1 ELISA Plate, 2x
    • Human Latent TGF-β1 Standard, 1 vial
    • Human Latent TGF-β1 Biotinylated Detection Antibody, 50 µL
    • SA-HRP, 30 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 120 mL
    • ELISA Diluent, 120 mL
    • SA-HRP Diluent, 25 mL
    • TMB Substrate, 25 mL
    • Stop Solution, 25 mL
    • Adhesive Plate Covers, 6x
 
  • Human Latent TGF-beta 1 ELISA Kit, 10 plates (Catalog #02019)
    • Human Latent TGF-β1 ELISA Plate, 10x
    • Human Latent TGF-β1 Standard, 1 vial
    • Human Latent TGF-β1 Biotinylated Detection Antibody, 250 µL
    • SA-HRP, 130 µL
    • Standard Reconstitution Buffer C, 1 mL
    • Wash Buffer (20X), 5 x 120 mL
    • ELISA Diluent, 3 x 120 mL
    • SA-HRP Diluent, 120 mL
    • TMB Substrate, 120 mL
    • Stop Solution, 120 mL
    • Adhesive Plate Covers, 30x
Subtype:
Complete Kits
Cell Type:
Dendritic Cells; Macrophages; Platelets; T Cells; Tumor Cells
Species:
Human
Area of Interest:
Immunology

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Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

Representative Standard Curve

• Reportable range: 0.32 - 32 pM. This is the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity. Recombinant human LAP (latency associated protein) homodimer is the analyte standard of this ELISA. Determination of latent TGF-β1 using the standard curve is based on a molar comparison. 1 pM LAP (54 pg/mL) corresponds to 1 pM latent TGF-β1 (80 pg/mL).
• Sensitivity: The limit of detection of this assay is 0.13 pM. This is the analyte concentration with absorbance two standard deviations higher than the zero standard.
• Recovery: A mid-curve recovery of 100% was determined by dilution of plasma samples in repeated experiments.
• Precision: The intra-assay precision of this assay is 3.0% (CV). The inter-assay precision of this assay is 10.4% (CV).

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.