Human ACE2 ELISA Kit

For detection and measurement of human ACE2

Human ACE2 ELISA Kit

For detection and measurement of human ACE2

From: 803 USD
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For detection and measurement of human ACE2
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What's Included

  • Human ACE2 ELISA Kit(Catalog #100-0XXX)
    • Human ACE2 ELISA Plate, 1x
    • Human ACE2 Standard, 2 vials
    • Human ACE2 Biotinylated Detection Antibody, 120 µL
    • SA-HRP, 120 µL
    • Human ACE2 Antibody Diluent, 15 mL
    • SA-HRP Diluent, 15 mL
    • ELISA Diluent, 50 mL
    • Wash Buffer (25X), 20 mL
    • TMB Substrate, 10 mL
    • Stop Solution, 10 mL
    • Adhesive Plate Covers, 4x

Overview

The Human ACE2 ELISA Kit is designed for the quantitative detection and measurement of human angiotensin converting enzyme 2 (ACE2) in biological fluids such as serum, plasma, and cell culture supernatant.

ACE2 is a type I integral membrane protein. It is highly expressed in capillary-rich organs such as the lungs, heart, blood vessels, kidneys, liver, and gastrointestinal tract. The ACE2 receptor is the target of the SARS-CoV-2 Spike (S) glycoprotein. This interaction allows for viral entry into the host cell, making ACE2 a potential target for antiviral therapeutics.

The assay is based on the sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for ACE2. The captured ACE2 is detected by addition of a biotinylated detection antibody, followed by streptavidin-horseradish peroxidase, which binds the biotinylated antibody. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of ACE2 in the sample. The concentration of ACE2 is determined by comparison to a serial dilution of the standard analyzed in parallel.
Application
ELISA
Area of Interest
Immunology, Infectious Diseases

Data Figures

Representative standard curve for Human ACE2 ELISA Kit

Figure 1. Representative Standard Curve

The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 0.156 - 10 ng/mL. The lower limit of detection (LLD) of this assay is 39 pg/mL. This is the lowest protein concentration that could be differentiated from zero as determined by the mean OD value of 20 replicates of the zero standard added by their three standard deviations. The intra- and inter-assay precision (CV) of this assay are < 8% and < 10%, respectively. This assay has high specificity for the detection of human ACE2. No significant cross-reactivity or interference is known to occur between human ACE2 and analogues.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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100-0687
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Safety Data Sheet 1
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100-0687
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Safety Data Sheet 2
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100-0687
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Safety Data Sheet 9
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100-0687
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