Mouse Recombinant TNF-alpha

Tumor necrosis factor alpha

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Tumor necrosis factor alpha
From: 202 USD

Overview

Tumor necrosis factor-α (TNF-α) is a pro-inflammatory cytokine that activates NF-κB, MAPK, and PI3K/AKT pathways. Activated T cells and macrophages are the primary producers of TNF-α in response to inflammation and infectious conditions. Many other cell types have been shown to produce TNF-α, among them B cells, NK cells, mast cells, neutrophils, dendritic cells, microglia, endothelial cells, smooth muscle cells, cardiomyocytes, and fibroblasts. TNF-α has cytotoxic effects on cancer cells in vitro by stimulating anti-tumor immunosuppressive responses. TNF-α stimulates expression of E- and P-selectins, thus facilitating adhesion of neutrophils, monocytes, and memory T cells to activated platelets and endothelial cells (Zelová & Hosek). Other effects of TNF-α include vasodilatation and edema formation.
Alternative Names:
Cachectin, Cachexin, Cytotoxin, DIF, Necrosin, TNF, TNF-α, TNFSF2, Tumor necrosis factor-α
Cell Type:
Airway Cells; Intestinal Cells; Hematopoietic Stem and Progenitor Cells; Neurons; B Cells; NK Cells
Species:
Mouse
Area of Interest:
Immunology; Stem Cell Biology; Epithelial Cell Biology; Neuroscience
Molecular Weight:
17.4 kDa
Purity:
≥ 95%

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Data and Publications

Data

(A) The biological activity of Mouse Recombinant TNF-alpha was tested by its ability to induce cytolysis of L929 cells growing in the presence of actinomycin D. Cell viability was measured after 19 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.013 0.02 ng/mL. (B) 1 μg of Mouse Recombinant TNF-alpha was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant TNF-alpha has a predicted molecular mass of 17.4 kDa.

STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485. PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.