Mouse Recombinant TECK (CCL25)

Thymus-expressed chemokine

Mouse Recombinant TECK (CCL25)

Thymus-expressed chemokine

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Thymus-expressed chemokine
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Overview

Thymus-expressed chemokine (TECK), or CCL25, is a member of the CC family of chemokines that regulates the movement of lymphocytes in the thymus and in the small intestine. TECK induces chemoattraction by binding the chemokine receptor CCR9, which is expressed on immature pre-T cells and thymocytes (Youn et al.; Uehara et al.). In the thymus, TECK is produced by stromal cells, whereas in the small intestine TECK is primarily produced by epithelial cells (Vicari et al.; Bowman et al.; Kunkel et al.). CCR9 is a G protein-coupled receptor and is expressed on most thymocytes, but not on natural killer cells, monocytes, eosinophils, basophils, and neutrophils (Wu et al). In Jurkat cells, binding of TECK to CCL9 has been shown to increase levels of intracellular calcium (Cheng-Rong et al). TECK/CCR9 signaling has also been linked to many cancers, as these molecules have been shown to mediate anti-apoptotic processes by activating the PI3K/AKT signaling pathway, weakening the effect of cytotoxic T cells by regulating STAT signaling. Additionally, TECK induces metastasis by increasing the expression of MMP2 and MMP9 (Tu et al.).
Subtype
Cytokines
Cell Type
Monocytes, T Cells, CD8+, T Cells, Other Subsets
Species
Mouse
Area of Interest
Immunology
Purity
≥ 98%

Data Figures

(A) The biological activity of Mouse Recombinant TECK (CCL25) was tested using a Ca2+ mobilization assay in CHO-K1 cells stably expressing Gα15 and CXCR3. Calcium mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which calcium mobilization is at 50% of maximum. The EC50 in the example above is less than 5 μg/mL. (B) 2 μg of Mouse Recombinant TECK (CCL25) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant TECK (CCL25) has a predicted molecular mass of 14.3 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78180.1, 78180
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78180.1, 78180
Lot #
All
Language
English

Applications

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