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Data and Publications
(A) The biological activity of Mouse Recombinant MIG (CXCL9) was tested using a Ca2+ mobilization assay in CHO-K1 cells stably expressing Gα15 and CXCR3. Calcium mobilization was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which calcium mobilization is at 50% of maximum. The EC50 in the example above is less than 2 μg/mL. (B) 2 μg of Mouse Recombinant MIG (CXCL9) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant MIG (CXCL9) has a predicted molecular mass of 12.3 kDa.