Data and Publications
(A) The biological activity of Mouse Recombinant IL-22 was tested by its ability to produce IL-10 in COLO 205 cells. IL-10 secretion was
measured after 4 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth
factor at which IL-10 production is at 50% of maximum. The EDC50 in the above example is 0.15 - 0.23 ng/mL.
(B) 1 μg of Mouse Recombinant L-22 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant IL-22 has a predicted molecular mass of 16.8 kDa.