Mouse Recombinant G-CSF

Granulocyte colony-stimulating factor

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Granulocyte colony-stimulating factor
From: 202 USD

Overview

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit et al.), the activation of metalloproteases that cleave VCAM-1 (Levesque et al.), and the release of norepinephrine from the sympathetic nervous system (Katayama et al.), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola et al.). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya et al.). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.
Alternative Names:
Colony-stimulating factor 3, CSF-3, MGI-1G, Pluripoietin
Cell Type:
Hematopoietic Stem and Progenitor Cells; Neurons; Cancer Cells and Cell Lines
Species:
Mouse
Area of Interest:
Stem Cell Biology; Neuroscience
Molecular Weight:
19.1 kDa
Purity:
≥ 95%

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(2)

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Data and Publications

Data

(A) The biological activity of Mouse Recombinant G-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell proliferation was measured after 69 hours of culture using a fluorometric assay method The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 8 - 12 pg/mL.
(B) 2 μg of Mouse Recombinant G-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant G-CSF has a predicted molecular mass of 19.1 kDa.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.