Mouse Recombinant G-CSF

Granulocyte colony-stimulating factor

Mouse Recombinant G-CSF

Granulocyte colony-stimulating factor

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Granulocyte colony-stimulating factor
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Overview

Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of glycoproteins that regulate hematopoietic cell proliferation, differentiation, and function. It is a key cytokine involved in the production of neutrophils and the stimulation of granulocyte colony formation from hematopoietic progenitor cells (Metcalf & Nicola). G-CSF causes a range of effects including a transient reduction of SDF-1 expression (Petit et al.), the activation of metalloproteases that cleave VCAM-1 (Levesque et al.), and the release of norepinephrine from the sympathetic nervous system (Katayama et al.), leading to the release or mobilization of hematopoietic stem cells from the bone marrow into the periphery. The G-CSF receptor is expressed on a variety of hematopoietic cells, including myeloid-committed progenitor cells, neutrophils, granulocytes, and monocytes. In addition to hematopoietic cells, G-CSF is also expressed in cardiomyocytes, neuronal cells, mesothelial cells, and endothelial cells. Mouse G-CSF was first purified from cultures of the WEHI-3B myelomonocytic leukemia cell line as the inducer of the terminal differentiation of WEHI-3B and other myeloid leukemia cell lines (Nicola et al.). It was later cloned in monkey COS cells from a cDNA library prepared with mRNA derived from mouse fibrosarcoma NFSA cells that produce G-CSF constitutively (Tsuchiya et al.). Binding of G-CSF to its receptor leads to activation of the JAK/STAT, MAPK, PI3K, and AKT signal transduction pathways.
Subtype
Cytokines
Cell Type
Cancer Cells and Cell Lines, Hematopoietic Stem and Progenitor Cells, Neurons
Species
Mouse
Area of Interest
Neuroscience, Stem Cell Biology
Purity
≥ 95%

Data Figures

(A) The biological activity of Mouse Recombinant G-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell proliferation was measured after 69 hours of culture using a fluorometric assay method The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 8 - 12 pg/mL.
(B) 2 μg of Mouse Recombinant G-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant G-CSF has a predicted molecular mass of 19.1 kDa.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
78014, 78014.1, 78014.2
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
78014, 78014.1, 78014.2
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Resources and Publications