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Data and Publications
(A) The biological activity of Mouse Recombinant G-CSF was tested by its ability to promote the proliferation of NFS-60 cells. Cell
proliferation was measured after 69 hours of culture using a fluorometric assay method The EC50 is defined as the effective
concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 8 - 12 pg/mL.
(B) 2 μg of Mouse Recombinant G-CSF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse Recombinant G-CSF has a predicted molecular mass of 19.1 kDa.