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Data and Publications
(A) The biological activity of Human Recombinant VEGF R2/KDR Fc was tested by the ability to inhibit VEGF-dependent HUVEC proliferation. Inhibition of cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is less than 0.03 μg/mL. (B) Human Recombinant VEGF R2/KDR Fc was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining.