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Data and Publications
(A) The biological activity of Human Recombinant TNF-receptor 1 was tested by its ability to neutralize human TNF-alpha induced cytolysis of L929 cells. Cell viability was measured after 66 hours using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell viability is at 50% of maximum. The EC50 in the above example is 0.01 μg/mL. (B) 1 μg of Human Recombinant TNF-receptor 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.