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Data and Publications
(A) The biological activity of Human Recombinant TNF-alpha, ACF was tested by its ability to inhibit growth of mouse L929 cells in the presence of actinomycin D. Inhibition of cell proliferation was measured after 48 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is 0.00877 ng/mL. (B) 1 μg of Human Recombinant TNF-alpha, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.