Human Recombinant TGF-beta 1

Transforming growth factor beta 1

More Views

From: 201 USD

Options

* Required Fields

Catalog # (Select a product)
Transforming growth factor beta 1
From: 201 USD

Overview

Transforming growth factor beta 1 (TGF-β1) is a member of the TGF-β superfamily and regulates diverse cellular phenotypes. TGF-β1 binds to serine-threonine kinase type I and II receptors and activates signal transduction through SMAD2/3 proteins, thus regulating a variety of functions, including cell proliferation, differentiation, wound healing, apoptosis, and metabolism (Massagué; McDowell et al.). During human embryogenesis, TGF-β1 is expressed by endothelial and hematopoietic tissues and acts as an endogenous autocrine growth regulator in those cells (Gatherer et al.). Together with basic fibroblast growth factor (bFGF), TGF-β1 supports the culture of undifferentiated human embryonic stem cells and induced pluripotent stem cells (Amit et al.). TGF-β1 is produced in latent form by many blood cells and is present in high amounts in platelets and bones. Once activated, TGF-β1 helps to maintain immune homeostasis by modulating B cell response and mediating immunosuppressive effects on T cells and neutrophils (Letterio & Roberts).
Alternative Names:
Cartilage-inducing factor, Differentiation inhibiting factor, Latency-associated peptide, TGFB1, TGF-beta-1, LAP, Transforming growth factor β 1
Cell Type:
Mesenchymal Stem and Progenitor Cells; Pluripotent Stem Cells; Neural Stem and Progenitor Cells; B Cells; T Cells, Regulatory; Monocytes; Myeloid Cells
Species:
Human
Area of Interest:
Stem Cell Biology; Immunology; Neuroscience
Molecular Weight:
12.8 kDa monomer, 25.6 kDa dimer
Purity:
≥ 95%

Scientific Resources

Product Documentation

Document Type
Product Name
Catalog #
Lot #
Language

Educational Materials

(8)

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant TGF-beta 1 was tested by the ability to inhibit IL-4-induced HT-2 cell proliferation. Cell proliferation was measured after 2 days of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 0.17 ng/mL. (B) 1 μg of Human Recombinant TGF-beta 1 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining.

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.