Human Recombinant SCF

Stem cell factor
Catalog #
78062_C
Stem cell factor
From: 185 USD

Overview

Stem cell factor (SCF) is an early-acting cytokine that plays a pivotal role in the regulation of embryonic and adult hematopoiesis. SCF promotes cell survival, proliferation, differentiation, adhesion, and functional activation of cells at multiple levels of the hematopoietic hierarchy. Together with other cytokines such as thrombopoietin and Flt3/Flk-2 Ligand, SCF is commonly used to promote expansion of primitive hematopoietic stem cells and multi-potent progenitor cells in culture (Martin et al.; Kent et al.). In synergy with various growth factors, including IL-2, IL-3, IL-6, IL-7, G-CSF, and erythropoietin, SCF increases proliferation and differentiation of myeloid and erythroid progenitor cells and a subset of lymphoid progenitor cells (Broudy). SCF is also a primary growth and activation factor for mast cells and eosinophils.

SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain.
Subtype
Cytokines
Alternative Names
Kit ligand, Mast cell growth factor, Steel factor, Stem cell factor
Cell Type
Hematopoietic Stem and Progenitor Cells, Mesoderm, PSC-Derived, Pluripotent Stem Cells
Species
Human
Area of Interest
Stem Cell Biology
Molecular Weight
18.6 kDa
Purity
≥ 95%

Related Products

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet 1
Product Name
Human Recombinant SCF
Catalog #
78062, 78062.1, 78062.2
Lot #
BX22769 and higher
Language
English
Document Type
Product Information Sheet 2
Product Name
Human Recombinant SCF
Catalog #
78062, 78062.1, 78062.2
Lot #
BX22769 and higher
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant SCF
Catalog #
78062, 78062.1, 78062.2
Lot #
All
Language
English

Educational Materials(2)

Brochure
Cytokines, Chemokines and Growth Factors
Brochure
Products for Human Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant SCF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1 ng/mL.
(B) 1 μg of Human Recombinant SCF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant SCF has a predicted molecular mass of 18.6 kDa.

Publications (1)

Nature communications 2014 jul Direct induction of haematoendothelial programs in human pluripotent stem cells by transcriptional regulators. I. Elcheva et al.

Abstract

Advancing pluripotent stem cell technologies for modelling haematopoietic stem cell development and blood therapies requires identifying key regulators of haematopoietic commitment from human pluripotent stem cells (hPSCs). Here, by screening the effect of 27 candidate factors, we reveal two groups of transcriptional regulators capable of inducing distinct haematopoietic programs from hPSCs: pan-myeloid (ETV2 and GATA2) and erythro-megakaryocytic (GATA2 and TAL1). In both cases, these transcription factors directly convert hPSCs to endothelium, which subsequently transform into blood cells with pan-myeloid or erythro-megakaryocytic potential. These data demonstrate that two distinct genetic programs regulate the haematopoietic development from hPSCs and that both of these programs specify hPSCs directly to haemogenic endothelial cells. In addition, this study provides a novel method for the efficient induction of blood and endothelial cells from hPSCs via the overexpression of modified mRNA for the selected transcription factors.
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