SCF exists in two biologically active splice forms: a soluble and a transmembrane isoform. Upon binding to its receptor (c-Kit tyrosine kinase receptor; CD117), it activates PI3K, JAK/STAT, and MAPK pathways. SCF and signaling from c-Kit have also been reported to play an important role in pigmentation, fertility, vasculogenesis, motility of the gut via c-Kit positive interstitial cells of Cajal, and in the migration of neuronal stem and progenitor cells to sites of injury in the brain.
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Data and Publications
(A) The biological activity of Human Recombinant SCF was tested by its ability to promote the proliferation of TF-1 cells. Cell proliferation
was measured after 72 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the
growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 1 ng/mL.
(B) 1 μg of Human Recombinant SCF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant SCF has a predicted molecular mass of 18.6 kDa.