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Data and Publications
(A) The biological activity of Human Recombinant PF-4 (CXCL4) was tested by its ability to inhibit human FGF-basic-dependent proliferation of mouse NR6R 3T3 cells. Cell proliferation was measured using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation inhibition is at 50% of maximum. The EC50 in the example above is less than 10 μg/mL. (B) 5 μg of Human Recombinant PF-4 (CXCL4) was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant PF-4 (CXCL4) has a predicted molecular mass of 7.8 kDa.