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Data and Publications
(A) The biological activity of Human Recombinant PDGF-AA was tested by its ability to promote the proliferation of BALB/c 3T3 cells. Cell proliferation was measured after 46 hours using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is less than 21 ng/mL. (B) 1 μg of Human Recombinant PDGF-AA was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant PDGF-AA is a homodimer of 14.4 kDa subunits with a predicted total molecular mass of 28.9 kDa.