Human Recombinant NT-4

Neurotrophin 4
Catalog #
78093_C
Neurotrophin 4
From: 212 USD

Overview

Neurotrophin-4 (NT-4) is a member of the nerve growth factor family which includes neurotrophin-3 (NT-3), brain-derived neurotrophic factor (BDNF), and nerve growth factor (NGF), all of which promote the differentiation, growth, and survival of peripheral and central nervous system neurons (Eide et al.). NT-4 binds and activates tropomyosin receptor kinase B (TrkB) at the cell surface; in doing so, it acts as a survival factor for certain populations of sensory neurons (Berkemeier et al.; Skaper). It has been shown that NT-4, together with BDNF, promotes neurite extension and maturation, as well as maintenance of differentiated cerebellar granule cells (Gao et al.).
Subtype
Cytokines
Alternative Names
GLC10, GLC1O, Neutrophic factor 4, Neutrophic factor 5, Neurotrophin-5, NT-4/5, NT-5, NTF4, NTF5
Cell Type
Astrocytes, Neural Cells, PSC-Derived, Neural Stem and Progenitor Cells, Neurons
Species
Human
Area of Interest
Neuroscience, Stem Cell Biology
Molecular Weight
14.0 kDa monomer; 28.1 kDa dimer
Purity
≥ 95%

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human Recombinant NT-4
Catalog #
78093
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant NT-4
Catalog #
78093
Lot #
All
Language
English

Educational Materials(4)

Brochure
Primary Neuronal Culture: Standardized Media and Reagents
Brochure
Cytokines, Chemokines and Growth Factors
Brochure
hPSC-Derived Neural Cell Research
Wallchart
Directed Differentiation of Pluripotent Stem Cells

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Data and Publications

Data

(A) The biological activity of Human Recombinant NT-4 was tested by its ability to promote the proliferation of a neuroblastoma cell line stably expressing TrkB (BR6). Cell proliferation was measured using a colorimetric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the above example is 5.6 ng/mL. For a representative bioactivity plot of C6 cell proliferation, contact us at techsupport@stemcell.com. (B) 1 μg of Human Recombinant NT-4 was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NT-4 is a homodimer of 14 kDa subunits with a predicted total molecular mass of 28.1 kDa.

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