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Data and Publications
(A) The biological activity of Human Recombinant NGF-beta was tested by its ability to promote the proliferation of TF-1 cells grown in GM-CSF free medium. Cell proliferation was measured after 67 hours of culture using a fluorometric assay method. The EC50 is defined as the effective concentration of the growth factor at which cell proliferation is at 50% of maximum. The EC50 in the example above is 0.54 ng/mL. (B) 1 μg of Human Recombinant NGF-beta was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant NGF-beta is a homodimer of 13.6 kDa subunits with a predicted total molecular mass of 27.3 kDa.