This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Data and Publications
(A) The biological activity of Human Recombinant MIP-1 alpha (CCL3) was tested by its ability to mobilize Ca2+ using CHO cells transfected with human CCR5, the receptor of human CCL3. Ca2+ mobilization was measured using a fluorometric FLIPR® assay method. The EC50 is defined as the effective concentration of the growth factor at which Ca2+ mobilization is at 50% of maximum. The EC50 in the example above is less than 0.08 μg/mL. (B) 1 μg of Human Recombinant MIP-1 alpha (CCL3) was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant MIP-1 alpha (CCL3) has a predicted molecular mass of 7.8 kDa.